REACTION OF ACTIVATED MONONUCLEATED CELLS TO CO-CULTIVATION WITH CALCIUM PHOSPHATE COATING OF TITANIUM MATRIX


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Abstract

The reactions of mononuclear blood cells (MNC) were evaluated under cocultivation with titanium substrates carrying a bilateral calcium phosphate coating (CP) under conditions of additional activation of the T-cell receptor. It has been established that in the activation 3D model, in contact with CP-coating, the secretion of pro- and anti-inflammatory cytokines (IL-2, TNF-α, IL-10, IL-4) increases, against the background of a decrease in the viability of the MNC culture and changes in their immunophenotype, in the direction of increasing the number of cells expressing CD95+ and CD45R0+ (the number of CD25+ cells has not changed), which may indicate that the MNC is hyperactive in the 3D model when it is stimulated by an activator of T cells.

About the authors

L. S. Litvinova

Immanuel Kant Baltic Federal University

Author for correspondence.
Email: fake@neicon.ru

MD, Head of the Basic Laboratory of Immunology and Cell Biotechnologies,

Kaliningrad

Russian Federation

K. A. Yurova

Immanuel Kant Baltic Federal University

Email: fake@neicon.ru

Ph.D., Researcher at the Base Laboratory of Immunology and Cell Biotechnology,

Kaliningrad

Russian Federation

V. V. Shupletsova

Immanuel Kant Baltic Federal University

Email: fake@neicon.ru

Ph.D. Researcher at the Base Laboratory of Immunology and Cell Biotechnology,

Kaliningrad

Russian Federation

O. G. Khaziakhmatova

Immanuel Kant Baltic Federal University

Email: fake@neicon.ru

Ph.D., Researcher at the Base Laboratory of Immunology and Cell Biotechnology,

Kaliningrad

Russian Federation

E. S. Melashchenko

Immanuel Kant Baltic Federal University

Email: lena.melashchenko17@mail.ru

Biologist of the Center for Medical Biotechnology,

Kaliningrad

Russian Federation

V. V. Malashchenko

Immanuel Kant Baltic Federal University

Email: fake@neicon.ru

Researcher, Center for Medical Biotechnologies,

Kaliningrad

Russian Federation

E. O. Shunkin

Immanuel Kant Baltic Federal University

Email: fake@neicon.ru

Engineer Researcher Basic Laboratory of Immunology and Cell Biotechnology,

Kaliningrad

Russian Federation

Yu. P. Sharkeev

Tomsk Polytechnic University

Email: fake@neicon.ru

Doctor of Physics and Mathematics, Professor, Head. lab Physics of nanostructured biocomposites,

Tomsk

Russian Federation

E. G. Komarova

Tomsk Polytechnic University

Email: fake@neicon.ru

Ph.D., Junior Researcher, Laboratory of Physics of Nanostructured Biocomposites,

Tomsk

Russian Federation

M. B. Sedelnikova

Tomsk Polytechnic University

Email: fake@neicon.ru

Doctor of Technical Sciences Senior Researcher, Laboratory of Physics of Nanostructured Biocomposites,

Tomsk

Russian Federation

I. K. Norkin

Immanuel Kant Baltic Federal University

Email: fake@neicon.ru

PhD student,

Kaliningrad

Russian Federation

K. I. Prokin

Immanuel Kant Baltic Federal University

Email: fake@neicon.ru

student,

Kaliningrad

Russian Federation

P. A. Ivanov

Immanuel Kant Baltic Federal University

Email: fake@neicon.ru

Researcher at the Base Laboratory of Immunology and Cell Biotechnology,

Kaliningrad

Russian Federation

I. A. Khlusov

Siberian State Medical University;
Tomsk Polytechnic University

Email: fake@neicon.ru

Ph.D. Professor of the Department of Morphology and General Pathology;

Professor of the Research School of Chemical and Biomedical Technologies,

Tomsk

Russian Federation

References

  1. Baht G. S., Vi L., Alman B. A. The Role of the Immune Cells in Fracture Healing. Curr Osteoporos Rep. 2018;16(2):138–145.

Copyright (c) 2019 Litvinova L.S., Yurova K.A., Shupletsova V.V., Khaziakhmatova O.G., Melashchenko E.S., Malashchenko V.V., Shunkin E.O., Sharkeev Y.P., Komarova E.G., Sedelnikova M.B., Norkin I.K., Prokin K.I., Ivanov P.A., Khlusov I.A.

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