Russian Journal of ImmunologyRussian Journal of Immunology1028-72212782-7291Russian Society of Immunology35710.31857/S102872210006680-3PHENOTYPING OF ANTIGEN-SPECIFIC CYTOTOXIC T-LYMPHOCYTES BY THE METHOD OF MULTICOLOR FLOW CYTOMETRYKuznetsovaM. S.<p>junior researcher of the Laboratory of Molecular Immunology,</p><p>Novosibirsk</p>fake@neicon.ruLopatnikovaJ. A.<p>PhD, senior researcher of the Laboratory of Molecular Immunology,</p><p>Novosibirsk</p>fake@neicon.ruSennikovS. V.<p>PhD, MD, professor, head of the Laboratory of Molecular Immunology, </p><p>Novosibirsk</p>sennikovsv@gmail.comResearch Institute of Fundamental and Clinical ImmunologyNovosibirsk State University15072019222-28378391606202016062020Copyright © 2019, Kuznetsova M.S., Lopatnikova J.A., Sennikov S.V.2019<p>Modern methods of multicolor flow cytometry in combination with MHC multimer technologies allow not only to identify and isolate cytotoxic T-lymphocyte (CTL) populations, but also to conduct phenotypic research on the level of differentiation, functional features of these cells, and even to investigate the distribution of memory T-cell subsets inside populations specific to particular epitopes of tumor antigens. In the present study, we assessed the level of differentiation and the content of memory T-cell subpopulations in cytotoxic T-lymphocyte populations specific for HER2/neu antigen epitopes (HER2) using the MHC-multimers antigen-specific CD8<sup>+</sup> T-cell staining method and multicolor flow cytometry. The resulting HER2-specific T cells to a large extent (about 40‒50%) have the TSCM phenotype, a population capable of the most pronounced antitumor immune response due to a combination of effector properties with self-sustainability. </p>cytotoxic T lymphocytesCD8+ T cellsmemory T-cell phenotypingmulticolor flow cytometryMHC multimersHER2/neuцитотоксические Т-лимфоцитыCD8+ Т-клеткифенотипирование Т-клеток памятимногоцветная проточная цитометрияМНС-мультимерыHER2/neu[1. Mahnke Y. D., Brodie T. M., Sallusto F., Roederer M., Lugli E. The who’s who of T-cell differentiation: Human memory T-cell subsets // Eur J Immunol 2013, 43, 2797–2809.][2. Кудрявцев И. В. Т-клетки памяти: основные популяции и стадии дифференцировки // Российский иммунологический журнал 2014, 8(17), 4, 947– 964.][3. Хайдуков С. В., Зурочка А. В., Тотолян А. А., Чере шнев В. А. Основные и малые популяции лимфоцитов периферической крови человека и их нормативные значения (методом многоцветного цитометрического анализа) // Медицинская Иммунология 2009, 11, 2–3, 227–238.][4. Kuznetsova M., Lopatnikova J., Khantakova J., Maksyutov R., Maksyutov A., Sennikov S. Generation of populations of antigen-specific cytotoxic T cells using DCs transfected with DNA construct encoding HER2/neu tumor antigen epitopes. // BMC Immunol 2017, 18, doi:10.1186/s12865-017-0219-7.]