Russian Journal of ImmunologyRussian Journal of Immunology1028-72212782-7291Russian Society of Immunology39010.31857/S102872210006548-7EFFECT OF MACROPHAGE CONDITION MEDIA ON PHENOTYPE AND PROLIFERATION OF HUMAN NEUROBLASTOMA CELL LINE SH-SY5YRashchupkinI. M.<p>second year residentof the Laboratory of Cellular Immunotherapy,</p><p>Novosibirsk</p>iwwwanbets@mail.ruTikhonovaM. A.<p>PhD (Biology), Senior Research Associate, Laboratory of Cellular Immunotherapy,</p><p>Novosibirsk</p>fake@neicon.ruTyrinovaT. V.<p>PhD (Medicine), Research Associate, Laboratory of Cellular Immunotherapy,</p><p>Novosibirsk</p>fake@neicon.ruSakhnoL. V.<p>PhD (Biology), Senior Research Associate, Laboratory of Cellular Immunotherapy,</p><p>Novosibirsk</p>fake@neicon.ruShevelaE. Ya.<p>PhD, MD (Medicine), Leading Research Associate, Laboratory of Cellular Immunotherapy, </p><p>Novosibirsk</p>fake@neicon.ruFederal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»15072019222-29039053006202030062020Copyright © 2019, Rashchupkin I.M., Tikhonova M.A., Tyrinova T.V., Sakhno L.V., Shevela E.Y.2019The study investigated the effect of conditioned media of M1 and M2 macrophages on the phenotype and proliferation of the human neuroblastoma cell line SH-SY5Y. It has been established that conditioned media of M2 macrophages, generated in the presence of GM-CSF under defi ciency of growth factors, have a pronounced stimulating effect on the SH-SY5Y cell proliferation. The expression of the low-affinity nerve growth factor receptor, CD271, correlates with cell morphology: in cultures with a predominance of N-type cells, the number of CD271+ cells is signifi cantly higher compared to cultures dominated by S-type cells.M1 and M2 macrophagesmacrophage-conditioned mediumneuroblastoma cell line SH-SY5YМ1 и М2 макрофагикондиционные средылиния нейробластомы человека SH-SY5Y[1. Hu X., Leak R.K., Shi J., Suenaga J., Gao Y., Zheng P., Chen J. Microglial and macrophage polarization – new prospects for brain repair. Nat Rev Neurol. 2015,11(1), 56–64.][2. Sakhno L.V., Shevela E.Ya., Tikhonova M. A., Ostanin A. A, Chernykh E. R. Scandinavian journal of Immunology. 2015. 83, 151–159.][3. Ferlemann F. C., Menon V., Condurat A. L., Rößler J., Pruszak J. Surface marker profiling of SH-SY5Y cells enables small molecule screens identifying BMP4 as a modulator of neuroblastoma differentiation. Sci Rep. 2017, 7(1), 1–14.]