Russian Journal of ImmunologyRussian Journal of Immunology1028-72212782-7291Russian Society of Immunology41210.31857/S102872210006535-3MODEL OF INFLUENCE HYPOSCIAS ON THE CELLULAR COMPONENT AND SYNTHESIS COMPONENTS EXTRACELLULAR MATRIX IN CULTURE FIBROBLASTSSuhovejU. G.<p>MD (Medicine), Professor, Director,</p><p>Tyumen</p>fake@neicon.ruKostolomovaE. G.<p>PhD (Biology), the assistant to faculty of pathological physiology,</p><p>Tyumen</p>lenakost@mail.ruUngerI. G.<p>PhD (Medicine), leading research associate,</p><p>Tyumen</p>fake@neicon.ruAkuneevaT. V.<p>senior research associate,</p><p>Tyumen</p>fake@neicon.ruAptekar’I. A.<p>PhD (Medicine), Director,</p><p>Tyumen</p>fake@neicon.ru«Tyumen filial of the Institute Clinical Immunology»FSBEI of Higher Education «Tyumen state medical university» of the Ministry
of Healthcare of Russian FederationANO «Tyumen institute of manual medicine»15072019222-29399412407202024072020Copyright © 2019, Suhovej U.G., Kostolomova E.G., Unger I.G., Akuneeva T.V., Aptekar’ I.A.2019It is established, that cultivation fibroblasts in conditions hypoxia leads to decrease in their morphological heterogeneity, increase proliferative to activity and viability of cells. Causes oppression of synthesis of components extracellular matrix (collagen and elastin) and decrease expression a receptor hyaluronic acids (CD44). Duration hypoxia causes a degree of decrease.hypoxiafibroblastsflow cytometrycollagenelastinгипоксияфибробластыпроточная цитометрияколлагенэластин[1. Шабанов П. Д., Зарубина И. В., Новиков В. Е., Цыган В. Н. Метаболические корректоры гипоксии. ‒ СПб., 2010. ‒ 916 с.][2. Dominici M., Le Blanc K., Mueller I., Slaper-Cortenbach I., Marini F., Krause D., Deans R., Keating A., Prockop D. J., Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4):315–7][3. Кудрявцев И. В., Хайдуков С. В., Зурочка А. В., Черешнев В. А. Применение метода проточной цитофлуориметрии для оценки пролиферативной активности клеток в медико-биологических исследованиях. Российский иммунологический журнал, 2012; 6(14), № 3–1:21–40.]