Russian Journal of ImmunologyRussian Journal of Immunology1028-72212782-7291Russian Society of Immunology52410.31857/S102872210007278-0POSTSTRESSOR CHANGES IN FUNCTIONAL ACTIVITY OF CELLS OF PERITONEAL CAVITY OF MICESharav’evaI. L.<p>PhD Biological Sciences, scientifi c researcher of the laboratory of biochemistry of microorganism development of the Institute of Ecology and Genetics of Microorganisms,</p><p>Perm</p>irin.sh@gmail.comGeinS. V.<p>MD, Professor, deputy director for research at the Institute of Ecology and Genetics of Microorganisms;</p><p>professor of the Department of microbiology and immunology of the Biology Faculty,</p><p>Perm</p>fake@neicon.ruInstitute of Ecology and Genetics of Microorganisms, UrB RASPerm State University15102019223130813121309202013092020Copyright © 2019, Sharav’eva I.L., Gein S.V.2019The effect of acute and chronic cold stress in the conditions of the blockade of opiate receptors on the oxidative activity of mouse peritoneal macrophages was studied. It was established that acute cold stress (4°C, 4 hours), regardless of the blockade of opiate receptors, inhibits zymosan-induced oxidative activity of mouse peritoneal macrophages. Chronic cold stress (4°C, 4 hours, for 7 days) on the 7th day activates zymosan-induced oxidative activity of macrophages, blockade of opiate receptors leads to the cancellation of this stimulation. An increase in the concentration of corticosterone is observed both in acute and in chronic hypothermia and does not depend on the blockade of opiate receptors.cold stresscorticosteronemacrophageschemiluminescenceopiate receptorsnaloxoneхолодовой стресскортикостеронмакрофагихемилюминесценцияопиатные рецепторыналоксон[1. McEwen B. C., Wingfield J. C. What’s in a name? Integrating homeostasis, allostasis and stress 2011, 57, 2, 1–16.][2. Поликарпов И. А., Кондратюк Е. Ю., Петровский Д. В., Новиков Е. А. Межпопуляционная изменчивость эндокринно-метаболической реакции на холодовой стресс у красной полевки (Myodes rutilus). Журнал общей биологии 2016, 77, 4, 284–292.][3. Sesti-Costa R., Baccan G. C., Chedraoui-Silva S., Mantovani B. Effects of acute cold stress on phagocytosis of apoptotic cells: the role of corticosterone Neuroimmunomodulation 2010, 17(2), 79–87.][4. Sugamaa S., Takenouchib T., Fujitac M., Kitanib H., Hashimotoc M. Cold stress induced morphological microglial activation and increased IL-1β expression in astroglial cells in rat brain. Journal of Neuroimmunology 2011, 233(1–2), 29–36.][5. Pancheri P., Zichella L., Fraioli F., Carilli L., Perrone G., Biondi M., Fabbri A., Santoro A., Moretti C. ACTH, beta-endorphin and met-enkephalin: peripheral modifications during the stress of human labor. Psychoneuroendocrinology 1985, 10(3), 289–301.][6. Suckow M. A., Terril L. A., Grigdesby C. F., March P. A. Evaluation of Hypothermia-Induced Analgesia and Infl uence of Opioid Antagonists in Leopard Frogs (Rana pipiens). Pharmacology Biochemistry and Behavior 1999, 63(1), 39–43.][7. Salmi P., Kela J., Arvidsson U., Wahlestedt C. Functional interactions between δ- and μ-opioid receptors in rat thermoregulation. European Journal of Pharmacology 2003, 458(1–2), 101–106.][8. Cadet P., Zhu W., Mantione K. J., Baggerman G., Stefano G. B. Cold stress alters Mytilus edulis pedal ganglia expression of mu opiate receptor transcripts determined by real-time RT-PCR and morphine levels. Brain Res Mol Brain Res 2002, 99(1), 26–33.][9. Sesti-Costa R., Ignacchiti M. D., Chedraoui-Silva S., Marchi L. F., Mantovani B. Chronic cold stress in mice induces a regulatory phenotype in macrophages: correlation with increased 11β-hydroxysteroid dehydrogenase expression. Brain Behav Immun 2012, 26(1), 50–60.][10. Zhu G. F., Chancellor-Freeland C., Berman A. S., Kage R., Leeman S. E., Beller D. I., Black P. H. Endogenous substance P mediates cold water stress-induced increase in interleukin-6 secretion from peritoneal macrophages. J. Neurosci 1996, 16(11), 3745–3752.][11. Гейн С. В., Шаравьева И. Л. Влияние холодового стресса на функциональную активность перитонеальных макрофагов мыши в условиях блокады опистных рецепторов. Российский физиологический журнал им. Сеченова 2016, 102, 188–194.][12. Иммунологические методы. Под ред. Г. Фримеля, М., 1987, 472.][13. Беседин И. М., Бажин А. С., Мельников В. П., Калёнова Л. Ф. Моделирующий эффект температурных воздействий слабой интенсивности на психонервноиммуноэндокринную систему теплокровного организма. Вестник Тюменского государственного университета. Экология и природопользование 2016, 2,2, 144–159.][14. Гейн С. В., Шаравьева И. Л. Иммуномодулирующие эффекты холодового стресса. Успехи современной биологии 2018, 138, 3, 243–250.][15. Salman H., Bergman M., Bessler H. Alexandrova S., Beilin B., Djaldetti M. Hypothermia affects the phagocytic activity of rat peritoneal macrophages. Acta Physiol Scand 2000, 68(3), 431–16.][16. Sandhu M. A., Zaib A., Anjum M. S., Qayyum M. Empirical evidence of cold stress induced cell mediated and humoral immune response in common myna (Sturnus tristis). Int J Biometeorol 2015, 59(11), 1607–13.][17. Козырева Т. В., Елисеева Л. С. Иммунный ответ и содержание кортикостероидов при различных режимах охлаждения. Бюллетень экспериментальной биологии и медицины 2002, 4, 384–387.]