Russian Journal of ImmunologyRussian Journal of Immunology1028-72212782-7291Russian Society of Immunology7410.31857/S102872210006581-4Research ArticleIDIOTYPIC AND ANTI-IDIOTYPIC ANTIBODIES AGAINST POLYCYCLIC AROMATIC HYDROCARBONS IN HUMAN BLOOD SERUM ARE NEW BIOMARKERS OF LUNG CANCERGrebenshchikovI. S.<p>Graduate Student, Leading Technologist in the Laboratory of Biotechnology, </p><p>Kemerovo</p>GrebenshchikovIS@ihe.sbras.ruStudennikovA. E.<p>Leading Technologist in the Laboratory of Biotechnology,</p><p>Kemerovo</p>fake@neicon.ruGlushkovA. N.<p>MD, Chef of the Institute of Human Ecology,</p><p>Kemerovo</p>fake@neicon.ruUstinovV. A.<p>PhD, Leader the Laboratory of Biotechnology,</p><p>Kemerovo</p>fake@neicon.ruFederal State Budget Scientific Institution «The Federal Research Center of Coal and Coal Chemistry of Siberian Branch of the Russian Academy of Sciences», Institute of Human Ecology15042019222-12212231504202015042020Copyright © 2019, Grebenshchikov I.S., Studennikov A.E., Glushkov A.N., Ustinov V.A.2019<p>We studied the levels of idiotypic and anti-idiotypic antibodies against benzo[a]pyrene in the blood serum of lung cancer patients and healthy donors by direct ELISA. It was shown that antibody levels between these groups was statistically signifi cantly different. Based on these results, a quantify antibodies immunoassay in the human serum was developed using a multiplex method.</p>Antibodypolycyclic aromatic hydrocarbonbenzo[a]pyreneBio-Plex 200lung cancerантителобензо[а]пиренполициклические ароматические углеводородыBio-Plex 200рак легкого[1. Kim K. H., Jahan S. A., Kabir E., Brown R. J. A review of airborne polycyclic aromatic hydrocarbons (PAHs) and their human health eff ects. Environ Int. 2013, 60, 71–80.][2. Ustinov V.A., Matveeva V.A., Kostyanko M.A., Glushkov A.N. Antibodies against benzo[a]pyrene in immunized mouse and in lung cancer patients. Exp Oncol. 2013, 35, 207–210.][3. Buratti M., Campo L., Fustinoni S., Cirla P.E., Martinotti I., Cavallo D., Foa V. Urinary hydroxylated metabolites of polycyclic aromatic hydrocarbons as biomarkers of exposure in asphalt workers. Biomarkers. 2007, 12(3), 221–239.][4. Zheng W, He L. Multiplexed Immunoassays. Advanced Techniques in Immunoassays published by Academic Pages, 5 Penn Plaza, 19th Floor, New York, NY10001, USA, 2017, 143–164.]