Russian Journal of ImmunologyRussian Journal of ImmunologyRussian Journal of Immunology1028-72212782-7291Russian Society of Immunology89910.31857/S102872210002415-1ORIGINAL ARTICLESОРИГИНАЛЬНЫЕ СТАТЬИSpontaneous and stimulated CD25 expression on T-lymphocytes and the amount of regulatory T-cells in children with chronic hepatitis CСпонтанная и стимулированная экспрессия СD25 Т-лимфоцитами и количество регуляторных Т-клеток у детей с хроническим гепатитом СPashninaI. .ПашнинаИ. А.
Russian Federation
Regional Children’s Clinical Hospital № 1Областная детская клиническая больницаInstitute of Immunology and Physiology, Urals Branch of the Russian Acad. Sci.Институт иммунологии и физиологии РАН1507201821338539022102020Copyright ©; 2018, Pashnina I...Copyright ©; 2018, Пашнина И.А.2018Pashnina I...Пашнина И.А.https://creativecommons.org/licenses/by/4.0https://rusimmun.ru/jour/article/view/899

Children of 6–17 years old with viral chronic hepatitis C (n=24) and conditionally healthy controls of corresponding age (n=32) were examined. The amount of CD3+CD25+, CD4+CD25+ и CD8+CD25+ and regulatory T-cells (Treg, CD3+CD4+CD25+CD127low/neg) were investigated by fl ow cytometry. In children with chronic hepatitis C the number of phytohemagglutinin-stimulated CD3+CD25+, CD4+CD25+ and Treg was decreased, the amount of CD8+CD25+ in conditions of stimulation by antibodies to CD3 and combined stimulation by antibodies to CD3 and CD28 was too decreased.

Обследованы дети 6–17 лет с хроническим вирусным гепатитом С (n=24), а также условно здоро- вые дети соответствующего возраста (контрольная группа, n=32). Методом проточной цитометрии исследовано количество CD3+CD25+, CD4+CD25+ и CD8+CD25+, регуляторных Т-клеток (Treg, CD3+CD4+CD25+CD127low/neg) в периферической крови и при инкубации с различными стимуляторами. У детей с хроническим гепатитом С по сравнению с контролем выявлено снижение количества CD3+CD25+, CD4+CD25+ и Treg при стимуляции фитогемагглютинином, а также CD8+CD25+ – при стимуляции антителами к CD3 и сочетанной стимуляции антителами к CD3 и CD28.

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