Russian Journal of ImmunologyRussian Journal of Immunology1028-72212782-7291Russian Society of Immunology97710.46235/1028-7221-977-IRTImmune reactions to clinical carcinogens and steroid hormones in breast pre-cancer and cancer patientsGlushkovA. N.<p><strong>Glushkov Andrew N</strong>., PhD, MD (Medicine), Professor, Chief Research Associate, Laboratory of Immunogenetics, Institute of Human Ecology</p><p><em>Kemerovo</em></p><p>SPIN-код (РИНЦ) 9536-8530;</p><p>AuthorID (Scopus) 7006323832;</p><p>ResearcherID (WOS) Q-5985-2016</p>glushkovan@ihe.sbras.ruhttps://orcid.org/0000-0002-8560-6719PolenokE. G.<p><strong>Polenok Elena G</strong>., PhD (Pharmacy), Leading Research Associate, Laboratory of Immunochemistry, Institute of Human Ecology, Federal Research Center of Coal and Coal Chemistry; Associate Professor, Department of Biology with the Basics of Genetics and Parasitology, Kemerovo State Medical University</p><p><em>Kemerovo</em></p><p>SPIN-код (РИНЦ) 3925-0185;</p><p>AuthorID (Scopus) 6506567994;</p><p>ResearcherID (WOS) Q-5381-2016</p>egpolenok@mail.ruhttps://orcid.org/0000-0002-9368-2340GordeevaL. A.<p><strong>Gordeeva Ludmila A</strong>., PhD (Biology), Leading Research Associate, Laboratory of Immunogenetics, Institute of Human Ecology</p><p><em>Kemerovo</em></p><p>SPIN-код (РИНЦ) 6662-4616;</p><p>AuthorID (Scopus) 14052058500;</p><p>ResearcherID (WOS) R-2781-2016</p>gorsib@rambler.ruhttps://orcid.org/0000-0001-5870-7584MunS. A.<p><strong>Mun Stella A.</strong>, PhD (Medicine), Senior Research Associate, Laboratory of Immunogenetics, Institute of Human Ecology</p><p><em>650065, Kemerovo, Leningradsky ave, 10</em></p><p>SPIN-код (РИНЦ) 5579-8939;</p><p>AuthorID (Scopus) 7101645456;</p><p>ResearcherID (WOS) J-6484-2018</p>stellamun@yandex.ruhttps://orcid.org/0000-0002-5530-3469KostyankoM. V.<p><strong>Kostyanko Mikhail V</strong>., Leading Engineer, Department of Organic Chemistry</p><p><em>Kemerovo</em></p><p>SPIN-код (РИНЦ) 5953-6554;</p><p>Author ID (Scopus) 6507008191</p><p><em> </em></p>kmvksu@mail.ruKolpinckiyG. I.<p><strong>Kolpinsky Gleb I</strong>., PhD, MD (Medicine), Professor, Department of Radiology, Radiotherapy and Oncology, Kemerovo State Medical University; Main Physician, Kemerovo Clinical Diagnostic Сenter</p><p><em>Kemerovo</em></p><p>SPIN-код (РИНЦ) 6894-6419,</p><p>AuthorID (Scopus) 56677706300</p>Glebss@mail.ruVafinI. A.<p>Chief Physician</p><p><em>Kemerovo</em></p>gkuz.kots@yandex.ruAntonovA. V.<p><strong>Antonov Alexander V</strong>., Head, Oncological Department No. 5</p><p><em>Kemerovo</em></p>al0412@mail.ruVerzhbitskajaN. E.<p><strong>Verzhbitskaya Natalia E</strong>., PhD (Medicine), Chief, Department of Pathology</p><p><em>Kemerovo</em></p>verzhbitskaja@mail.ruInstitute of Human Ecology, Federal Research Center of Coal and Coal ChemistryKemerovo State Medical UniversityKemerovo State UniversityKemerovo Clinical Diagnostic СenterKuzbass Center of BloodKuzbass Clinical Oncology Dispensary1501202124110110804122020Copyright © 2021, Glushkov A.N., Polenok E.G., Gordeeva L.A., Mun S.A., Kostyanko M.V., Kolpinckiy G.I., Vafin I.A., Antonov A.V., Verzhbitskaja N.E.2021<p>Detection of postmenopausal women at high risk for breast pre-cancer and cancer is a key condition to prevent these diseases. Aim of our research was to study possible usage of immunoassay for antibodies specific to benzo[a]pyrene, estradiol, and progesterone (IgA-Bp, IgA-Es, IgA-Pg) in determination of personal risks for fibrocystic disease and breast cancer at the early stage, with respect to hormone receptor status in tumor tissues. Blood serum IgA-Bp, IgA-Es, IgA-Pg were studied by ELISA in postmenopausal women: healthy controls (n = 401), patients with fibrocystic breast disease (n = 50), and breast cancer (stage I, n = 575, stages II-IV, n = 861). High individual IgA-Bp/IgA-Pg ratios of 1.5 were found in 19.7% of healthy women, and in 50.0% of fibrocystic breast disease patients (p 0.0001; OR = 4.1). IgA-Es/IgA-Pg ratios of 1.0 were revealed in 48.4% healthy women and in 68.0% fibrocystic breast disease patients (p 0.01; OR = 2.3). IgA-Bp/IgAPg values 1.0 were found in 41.9% of healthy women, and, at higher rates, in the patients with breast cancer stage I: 68.3% ER- tumors (p 0.0001; OR = 3.0) and 75.9% ER+ tumors (p 0.0001; OR = 4.4). IgA-Es/ IgA-Pg ratios 1.0 were revealed in 48.4% of healthy women, and in patients with breast cancer stage I: 65.3% ER- tumors (p 0.003; OR = 2.0), and 76.8% ER+ tumors (p 0.0001; OR = 3.5). Some associations of studied antibodies with cancer progression were revealed. Frequency of individual cases with IgA-Bp/IgA-Pg 1.0 in patients with ER- tumors increased from 12.0% at stage I to 19.9% at stage II. Frequency of cases with IgA-Bp/IgA-Pg 1.0 in the patients with ER+ tumors decreased from 62.0% at stage I to 57.3% at stage II (p = 0.002). Frequency of cases with IgA-Es/IgA-Pg 1.0 in the patients with ER- tumors increased from 11.5% at stage I to 21.4% at stage II. Frequency of cases with IgA-Es/IgA-Pg 1.0 in patients with ER+ tumors decreased from 63.3% at stage I to 56.1% at stage II (p 0.001). The cases with individual excessive IgA-Bp and IgA-Es levels are associated with fibrocystic breast disease and ER+ breast cancer at the onset of the disease. Breast cancer progression was associated with the relative decrease of ER in tumor tissues, along with higher individual levels of IgA-Bp and IgА-Es and lower IgA-Pg levels. ELISA testing of IgА-Bp, IgА-Es, IgA-Pg could be recommended for detection of individual risk for fibrocystic breast disease and stage I of breast cancer, as well as for more efficient prevention and therapy by selective modulators of estrogen receptor (raloxifene, arzoxifene and lasofoxifine) and aromatase inhibitors (exemestane, anastrozole).</p>antibodiesbenzo[a]pyreneestradiolprogesteronefibrocystic diseasebreast cancersteroids receptorsантителабензо[а]пиренэстрадиолпрогестеронфиброзно-кистозная болезньрак молочной железыстероидные рецепторы[1. Глушков А.Н., Поленок Е.Г., Мун С.А., Гордеева Л.А., Костянко М.В., Колпинский Г.И., Луценко В.А., Антонов А.В., Титов В.А., Вафин И.А. Антитела к химическим канцерогенам и половым стероидам и содержание эстрадиола и прогестерона в сыворотке крови женщин больных раком молочной железы и мужчин больных раком легкого // Российский иммунологический журнал, 2020. Т. 23, № 1. С. 69-78.][2. 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