Relationships between spermogram indexes in males with chronic bacterial prostatitis and the levels of local antimicrobial factors

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Abstract

Sperm quality can be directly or indirectly affected in chronic bacterial prostatitis, due to protein components of mucosal immunity, including lactoferrin, secretory immunoglobulin A, and lysozyme. Therefore, a search for relationships between spermogram indices and levels of antimicrobial factors in sperm plasma presents an urgent task in healthy men and patients with chronic bacterial prostatitis. The present paper contains the results of a study of sperm samples from 72 men aged 20 to 45 years. Samples were collected in sterile containers by masturbation after a minimum abstinence period of 3-5 days. None of the patients had previously taken antibiotics. Patients with sexually transmitted infections were excluded from the study. The patients were divided into two groups — conditionally healthy males (n = 30) and patients with chronic bacterial prostatitis (n = 42). Seminal plasma was obtained by two-stage centrifugation of ejaculate samples (1000-3000 rpm) for 30 minutes. Evaluation of lactoferrin and secretory immunoglobulin A levels in seminal plasma was carried out by ELISA technique (Vector-Best, Novosibirsk), lysozyme — by turbidimetric method. The results were recorded with Multiscan Labsystems photometer (Finland) at 492-nm wavelength. Statistical evaluation of the obtained data was carried out using Statistica 10 package (StatSoft, USA). To assess the kind of relationships between the studied parameters, the Spearman's rank correlation quotient was applied. When using the Spearman rankcorrelation approach, the strength of the relationships between the features was assessed, considering the values of < 0.3 as a weak connection; quotient levels of 0.4 to 0.7 as indexes of moderate relationships, and values of 0.7 and more, as high connection indexes.

We have found that an increased level of lactoferrin in some cases of leukocytospermia, oligospermia, and asthenospermia is accompanied by decreased number of leukocytes, increased sperm motility, preservation of sperm morphology, thus suggesting improvement of the sperm quality. Considering universal biological properties of lactoferrin and its receptors, an increased number of positive correlations between level lactoferrin level and spermogram parameters was shown in the patients with chronic bacterial prostatitis, as compared with healthy males, thus presuming high diagnostic value of this marker. Hence, diagnostic value of spermogram parameters is determined not only by their quantitative values, but also by the types of correlations with antimicrobial factor levels, in particular, with those of lactoferrin, secretory immunoglobulin A and lysozyme.

About the authors

A. V. Bekpergenova

Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Email: nsavasteeva@gmail.com

PhD (Biology), Senior Research Associate, Laboratory of Infectious Symbiology, Institute of Cellular and Intracellular Symbiosis, Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences.

460000, Orenburg, Pionerskaya str., 11.

Russian Federation

T. A. Bondarenko

Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Email: semenovih88@mail.ru

Research Associate, Laboratory of Infectious Symbiology, Institute of Cellular and Intracellular Symbiosis, Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences.

460000, Orenburg, Pionerskaya str., 11.

Russian Federation

N. B. Perunova

Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Author for correspondence.
Email: perunovanb@gmail.com

Natalia B. Perunova - PhD, MD (Medicine), Professor of RAS, Leading Research Associate, Head, Laboratory of Infectious Symbiology, Institute of Cellular and Intracellular Symbiosis, Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences.

460000, Orenburg, Pionerskaya str., 11.

Phone: 7 (922) 555-30-80.

Russian Federation

O. E. Chelpachenko

Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Email: oech57@gmail.com

PhD, MD (Medicine), Professor, Leading Research Associate, Laboratory of Infectious Symbiology, Institute of Cellular and Intracellular Symbiosis, Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences.

460000, Orenburg, Pionerskaya str., 11.

Russian Federation

I. A. Nikiforov

Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences

Email: ianikiforov@rambler.ru

PhD (Geology/Mineralogy), Leading Research Associate, Laboratory of Infectious Symbiology, Institute of Cellular and Intracellular Symbiosis, Institute of Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences.

460000, Orenburg, Pionerskaya str., 11.

Russian Federation

References

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  5. Silva J.A.F., Biancardi M.F., Stach-Machado D.R., Reis L.O., Sant'Anna O.A., Carvalho H.F. The origin of prostate gland-secreted IgA and IgG. Sci. Rep., 2017, Vol. 7, no. 1, 16488. doi: 10.1038/s41598-017-16717-3.
  6. Wang P., Liu B., Wang Z., Niu X., Su S., Zhang W., Wang X. Characterization of lactoferrin receptor on human spermatozoa. Reprod. Biomed. Online, 2011, Vol. 22, no. 2, pp. 155-161.
  7. World Health Organization (WHO). WHO laboratory manual for the examination and processing of human semen. 5th. Geneva, Switzerland: World Health Organization; 2010.

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Copyright (c) 2021 Bekpergenova A.V., Bondarenko T.A., Perunova N.B., Chelpachenko O.E., Nikiforov I.A.

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Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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