Study of relationships between maternal HLA-G gene polymorphism and intrauterine infection with risk of congenital malformations

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Abstract

This study aims for assessing relationships between maternal HLA-G gene polymorphisms (rs41551813, rs12722477, rs41557518) and intrauterine infection with the risk of congenital malformations (CM) in infants. We studied 331 women who had offspring with CMs, and 408 women with one or more healthy children. Influence of the intrauterine infection was analyzed on the basis of laboratory tests. Diagnostics of bacterial vaginosis and vulvovaginal candidiasis by microscopic examination were conducted. Viral infections (herpes simplex virus type 2, cytomegalovirus, human papilloma virus type 16/18) as well as Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, Gardnerella vaginalis; Trichomonas vaginalis and Toxoplasma gondii were detected by enzyme-linked immunoassay or polymerase chain reaction (PCR) techniques. The data were obtained from the medical cards of the surveyed women. The gene polymorphisms were typed for Thr31Ser (rs41551813, HLA-G*01:03) in exon 2, Leu110Ile (rs12722477, HLA-G*01:04) and 1597 delС (rs41557518, HLA-G*01:05N) in exon 3 HLA-G using real-time PCR followed by melting analysis. The study showed that maternal age was not a significant risk factor for CMs in the fetus/newborns. Meanwhile, the maternal intrauterine infections were shown to be a significant risk factor for CMs in their infants (OR = 1.57 (1.08-2.29); p = 0.002). It was found that the 110 Ile allele (HLA-G *01:04) was a risk factor for CMs incidence in the fetus/newborns (OR = 1.57 (1.08-2.29), p = 0.01). No association was found between the maternal rs41551813 and rs41557518 HLA-G genetic polymorphisms and CMs in the infants. Hence, intrauterine infections and maternal 110 Ile allele (HLA-G *01:04) may be suggested as risk factors for birth defects in the children. Our results will be useful in understanding the molecular mechanisms of immune disorders in feto-maternal interface.

About the authors

L. A. Gordeeva

Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences

Author for correspondence.
Email: gorsib@rambler.ru
ORCID iD: 0000-0001-5870-7584

PhD (Biology), Leading Research Associate, Laboratory of Immunogenetics

650065, Kemerovo, Leningradsky ave., 10

Russian Federation

E. N. Voronina

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences

Email: voronina_l@mail.ru

PhD (Biology), Research Associate, Molecular Genetics Group

Novosibirsk

Russian Federation

Yu. V. Gareeva

LLC "Medical Practice"

Email: gareeva.j@bk.ru

Clinical Neonatologist

Kemerovo

Russian Federation

E. G. Polenok

Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences

Email: egpolenok@mail.ru

PhD (Pharmacy), Leading Research Associate, Laboratory of Immunochemistry

650065, Kemerovo, Leningradsky ave., 10

Russian Federation

S. A. Mun

Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences

Email: stellamun@yandex.ru

PhD (Medicine), Senior Research Associate, Laboratory of Immunogenetics

650065, Kemerovo, Leningradsky ave., 10

Russian Federation

A. N. Glushkov

Federal Research Center of Coal and Coal Chemistry, Siberian Branch, Russian Academy of Sciences

Email: ihe@kemtel.ru

PhD, MD (Medicine), Deputy Director

650065, Kemerovo, Leningradsky ave., 10

Russian Federation

References

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Copyright (c) 2021 Gordeeva L.A., Voronina E.N., Gareeva Y.V., Polenok E.G., Mun S.A., Glushkov A.N.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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