CD36 expression intensity on different subpopulations of circulating monocytes depending on the extent of subclinical atherosclerosis

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Abstract

It is known that chronic activation of innate immunity and persistent low-intensity inflammation play a crucial role in the initiation and progression of atherosclerosis. It was found that atherogenic lipoproteins can act as inducers of the inflammatory response through ligand-receptor interaction with pattern-recognizing receptors of immunocompetent cells, such as CD36 (SR-B2) and Toll-like receptors. It is suggested that expression of CD36 on circulating monocytes may represent the burden of systemic atherosclerosis and, therefore, act as its diagnostic marker. The aim of the present study was to assess the intensity of CD36 expression on circulating monocytes of different subpopulations in patients without established cardiovascular disease (CVD) depending on the extent of subclinical atherosclerosis of peripheral arteries. One hundred patients without established atherosclerotic CVD, 49 (49.0%) men and 51 (51.0%) women, were included in the study. Monoclonal antibody conjugates were used to phenotype monocyte subpopulations. The expression of CD36 on CD14++CD16- monocytes (classical monocytes), CD14+CD16+ monocytes (intermediate monocytes), CD14+CD16++ monocytes (non-classical monocytes) was determined by the average fluorescence intensity. There was a statistically significant decrease in CD36 expression intensity on classical and non-classical monocytes with increasing number of vascular basins affected by atherosclerosis. A statistically significant decrease in CD36 expression intensity on classical and non-classical monocytes was found in the patients with two vascular beds lesions in comparison with patients with a single vascular bed lesion upon pairwise comparisons.

About the authors

I. I. Dolgushin

South-Ural State Medical University

Email: dol-ii@mail.ru
ORCID iD: 0000-0002-0901-8042

 PhD, MD (Medicine), Professor, Full Member, Russian Academy of Sciences, President, Head, Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics, Director, Research Institute of Immunology 

454092, Russian Federation, Chelyabinsk, Vorovsky str., 64

Russian Federation

V. V. Genkel

South Ural State Medical University

Author for correspondence.
Email: henkel-07@mail.ru
ORCID iD: 0000-0001-5902-3803

PhD (Medicine), Associate Professor, Department for Propaedeutics of Internal Diseases 

454092, Russian Federation, Chelyabinsk, Vorovsky str., 64

Phone: 7 (951) 441-70-61 

Russian Federation

I. L. Baturina

South Ural State Medical University

Email: irisha_baturina@mail.ru
ORCID iD: 0000-0002-5960-4189

 PhD (Medicine), Senior Research Associate, Research Institute of Immunology 

454092, Russian Federation, Chelyabinsk, Vorovsky str., 64

Russian Federation

A. Yu. Savochkina

South Ural State Medical University

Email: alina7423@mail.ru
ORCID iD: 0000-0002-0536-0924

 PhD, MD (Medicine), Professor, Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics, Chief Research Associate, Research Institute of Immunology 

454092, Russian Federation, Chelyabinsk, Vorovsky str., 64

Russian Federation

A. A. Minasova

South Ural State Medical University

Email: pandora_anna@mail.ru
ORCID iD: 0000-0002-9084-0577

 PhD (Biology), Associate Professor, Department of Microbiology, Virology, Immunology and Clinical Laboratory Diagnostics 

454092, Russian Federation, Chelyabinsk, Vorovsky str., 64

Russian Federation

A. S. Kuznetsova

South Ural State Medical University

Email: kuzja321@mail.ru
ORCID iD: 0000-0002-1136-7284

 PhD (Medicine), Associate Professor, Department of Hospital Therapy 

454092, Russian Federation, Chelyabinsk, Vorovsky str., 64

Russian Federation

I. I. Shaposhnik

South Ural State Medical University

Email: shaposhnik@yandex.ru
ORCID iD: 0000-0002-7731-7730

 MD, PhD (Medicine), Professor, Head, Department for Propaedeutics of Internal Diseases 

454092, Russian Federation, Chelyabinsk, Vorovsky str., 64

Russian Federation

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Copyright (c) 2021 Dolgushin I.I., Genkel V.V., Baturina I.L., Savochkina A.Y., Minasova A.A., Kuznetsova A.S., Shaposhnik I.I.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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