CHANGES IN THE INTERFERONAL STATUS INDICATORS IN PATIENTS OF THE LOCAL-DISTRIBUTED KIDNEY CANCER IN THE DYNAMICS OF THE DISEASE

The aim of the research was to study the indicators of serum α- (IFN-α) and γ-interferon (IFN-γ), the levels of spontaneous and stimulated production of interferons by peripheral blood mononuclear cells in patients with locally advanced renal cell carcinoma (RCC) before and 14 days after the operation. Patients with RCC (T3 N0 M0) were examined before surgical treatment and 14 days after radical nephrectomy. The concentration of IFN-α and IFN-γ in serum was determined using enzyme-linked immunosorbent assay (ELISA). The level of spontaneous and stimulated (concanavalin A) production of IFN-α and IFN-γ was determined in the supernatants of peripheral blood mononuclear cells cultured for 72 hours by ELISA. It was found that in patients with locally advanced RCC, both before surgery and after surgical treatment, an increase in serum levels of IFN-α and IFN-γ was observed, and the spontaneous production of IFN-γ by peripheral blood mononuclear cells was also increased. 14 days after surgery there was a decrease in the spontaneous production of IFN-α in combination with an increased ability of peripheral blood mononuclear cells to induce the production of IFN-α and IFN-γ. The identified imbalance in the IFN-producing system in patients with RCC in the postoperative period must be considered when developing immunorehabilitation programs in this category of patients.

cancer, kidney, interferon, mononuclear cells, peripheral blood