DEPENDENCE OF THE BLOOD T-LYMPHOCYTES PHENOTYPE FROM THE OUTCOME OF THE WIDESPREAD PURULENT PERITONITIS


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Abstract

The purpose of the research was to study the phenotypic composition of peripheral blood T-lymphocytes in humans in the dynamics of postoperative treatment depending on the outcome of the widespread purulent peritonitis (WPP). 38 patients with acute surgical diseases and abdominal cavity injuries complicated by WPP were examined. Blood sampling was performed before the operation (preoperative period) and also on the 7th, 14th and 21st day of the postoperative period. The study of the phenotype of blood T-lymphocytes was carried out by flow cytometry. It was established that the phenotype of blood T-lymphocytes in patients with WPP in the pre- and post-operative period differs significantly in depending on the outcome of the disease. With a favorable outcome of WPP in the pre-operative period, an increased number of common T-lymphocytes, including expression of CD62L, and CD4+-T cells, primarily due to Treg (including activated cells) are detected. Consequently, an increase in the amount of Treg in patients with WPP at the peak of the disease is a prognostic sign of a favorable outcome. Treg regulate the intensity of the inflammatory process that leads to an improvement in the clinical state of patients with WPP and accordingly increases the likelihood of a favorable outcome of the disease. The phenotype of blood T-lymphocytes significantly changes in the dynamics of post-operative treatment. Regardless of the outcome of the disease, the content of T-cells expressing CD28 and CD62L, as well as the T-helper and Treg level, is significantly reduced in patients. Only with a favorable outcome of the disease at the end of the observed period (21 days of treatment) in the blood increases the amount of cytotoxic T-lymphocytes and T-cells with CD57 expression. These changes in the phenotypic composition of T-lymphocytes are determined by the use of antibiotics and the migration of cells into the inflammation zone. 

About the authors

V. D. Belenjuk

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of medical problems of the North

Author for correspondence.
Email: fake@neicon.ru

junior researcher, Laboratory of the Cellular Molecular Physiology and Pathology, 

Krasnoyarsk

Russian Federation

A. A. Savchenko

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of medical problems of the North;
Siberian Federal University

Email: aasavchenko@yandex.ru

doctor of medicine, professor, head of the laboratory of the Cellular Molecular Physiology and Pathology;

Department of Medical Biology,

660022, Krasnoyarsk, P. Dzelezniaka str, 3Г

Russian Federation

A. G. Borisov

Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of medical problems of the North

Email: fake@neicon.ru

PhD, leading researcher, laboratory of the Cellular Molecular Physiology and Pathology,

Krasnoyarsk

Russian Federation

I. V. Kudryavtsev

Federal State Budgetary Scientific Institution “Institute of Experimental Medicine”

Email: fake@neicon.ru

PhD (Biology), senior research associate, department of Immunology,

St. Petersburg

Russian Federation

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Copyright (c) 2019 Belenjuk V.D., Savchenko A.A., Borisov A.G., Kudryavtsev I.V.

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