A role of single nucleotide polymorphism in TNF, NOS3 and MMP9 genes at the risk of developing neonatal pneumonia

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Neonatal pneumonia is one of the most common causes of infant mortality. Moreover, the diagnosis of this pathology represents a difficult task and requires to seek for additional solutions in clinical and laboratory practice. One of the current and promising ways to diagnose rerlies on search of predictive markers among the innate and adaptive immunity genes involved in disease pathogenesis. We used bioinformatics analysis to select genes playing essential role developing neonatal pneumonia. Next, cord blood samples collected from 234 newborns were examined by real-time polymerase chain reaction for nine polymorphic markers in the TNF, IL10, IL17A, IL17F, IL6, NOS3, and MMP9 genes. The results of statistical analysis showed that heterozygous genotypes in the MMP9 and TNF genes were associated with the risk of developing early neonatal pneumonia, and also the protective role of homozygotes AA in the MMP9 gene and GG in the TNF gene. A search for associations with a risk of intrauterine pneumonia revealed an unfavorable role for heterozygous genotypes in the NOS3 and MMP9 genes. Thus, due to difficulties in diagnosis or in case of developing neonatal pneumonia, it may be recommended to add genetic analysis for assessing polymorphic markers in the MMP9 (rs17576), TNF (rs1800629) and NOS3 (rs1549758) genes along with standard test assays.

About the authors

E. A. Meremianina

I. Mechnikov Research Institute for Vaccines and Sera; Russian Medical Academy of Continuing Professional Education

Author for correspondence.
Email: ekatterine@gmail.com

Meremianina Ekaterina A. - PhD (Medicine), Junior Research Associate, Laboratory of Molecular Immunology; Senior Lecturer, Department of Virology

105064, Moscow, Maly Kazenny lane, 5a

Phone: 7 (926) 184-14-89

Russian Federation

O. A. Svitich

I. Mechnikov Research Institute for Vaccines and Sera; First Moscow State I. Sechenov Medical University (Sechenov University)

Email: fake@neicon.ru

PhD, MD (Medicine), Corresponding Member, Russian Academy of Sciences, Professor, Department of Microbiology, Virology and Immunology; Director


Russian Federation

A. I. Alieva

Dagestan State Medical Academy

Email: fake@neicon.ru

PhD, MD (Medicine), Associate Professor, Dean, Faculty of Medicine and Prevention

Makhachkala, Republic of Dagestan

Russian Federation

V. V. Sobolev

I. Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

PhD (Biology), Senior Research Associate, Laboratory of Molecular Immunology


Russian Federation


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Copyright (c) 2020 Meremianina E.A., Svitich O.A., Alieva A.I., Sobolev V.V.

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