Features of immune regulation and specific sensitization in children of the Northern Siberia

Cover Page

Cite item


This paper highlights the features of immune regulation and specific hypersensitivity in children residing in the Northern Siberia being exposed to the extreme climatic and exogenous chemical risk factors. An immunological diagnostic examination of 255 children permanently residing in the region of exogenous hapten exposure was conducted. The control group consisted of 130 children residing on the territory not being exposed to exogenous chemical factors. Level of serum class G, A, M immunoglobulins was assessed by using a Mancini radial immunodiffusion reaction. Amount of total serum IgE, neuron-specific enolase, and VEGF was measured by using the ELISA. Changes in level of IgG specific to phenol and copper as well as IgE specific to nickel and benz(a)pyrene were measured by using enzyme-linked allergosorbent test. Peripheral blood lymphocyte subsets were analyzed by using membrane-bound immunofluorescence with a panel of labeled specific monoclonal antibodies. High-quality statistical data analysis was performed by using Microsoft® Office Excel 2003 software as well as Statistica 6.0 application software package (StatSoft, USA). Statistical significance was determined by using Student’s t-test. Relation between signs was estimated by using correlation and regression analysis as well as Fisher’s test. Immunological study demonstrated that immunogram parameters were significantly changed in the observation group by containing increased percentage of CD16+ effector cells absolute and relative CD19+ cell count (p ≤ 0.05), relative and absolute count of CD25+ lymphocytes (p ≤ 0.05) as well as CD95+T cells as compared to the reference values and control group (p ≤ 0.05). Moreover, level of neuron-specific enolase and VEGF (vascular endothelial growth factor) was increased compared to control group (p ≤ 0.05). It points that regulatory mediators potentially mapped to the glia and vascular intima in children from the observation group were extremely activated. The level of both total (total IgE) and specific sensitization was significantly increased by assessing immunoglobulin G specific to phenol as well as immunoglobulin E specific to nickel and benz(a)pyrene (p < 0.05). Parameters of programmed cell death were significantly altered that was paralleled with simultaneous activation of general and specific sensitization highlighting features of the immunological status in children examined distinguished by excessive stress of immune regulatory arms in case of combined exposure to climate-geographic as well as exogenous chemical risk factors.

About the authors

Yu. A. Chelakova

Federal Research Center for Medical and Preventive Health Risk Management Technologies

Author for correspondence.
Email: ChelakovaYu@yandex.ru

Chelakova Yulia A. - Junior Research Associate, Laboratory of Immunology and Allergology

614045, Perm, Monastyrskaya str., 82

Phone: 7 (922) 380-03-38

Russian Federation


  1. Белоусова Н.А. Шибков А.А., Байгужин П.А. Анализ состояния здоровья детей и подростков, проживающих в условиях промышленного мегаполиса // Вестник Российского университета дружбы народов. Серия: Экология и безопасность жизнедеятельности, 2013. № 2. С. 38-43. [Belousova N.A., Shibkov A.A., Bayguzhin P.A. A medical examination of children and adolescents living in industrial cities. Vestnik Rossiyskogo universiteta druzhby narodov. Seriya: Ekologiya i bezopasnost zhiznedeyatelnosti = Bulletin of the Peoples’ Friendship University of Russia. Series: Ecology and Life Safety, 2013, no. 2, pp. 38-43. (In Russ.)]
  2. Зайцева Н.В., Ланин Д.В., Черешнев В.А. Иммунная и нейроэндокринная регуляция в условиях воздействия химических факторов различного генеза. Пермь: Изд-во Перм. нац. исслед. политехн. ун-та, 2016. 236 с. [Zaitseva N.V., Lanin D.V., Chereshnev V.A. The immune and neuroendocrine regulation in the conditions of influence of chemical factors of various origins]. Perm: Perm National Research Polytechnic University Press, 2016. 236 p.
  3. Ланин Д.В., Зайцева Н.В., Землянова М.А., Долгих О.В., Дианова Д.Г. Характеристика регуляторных систем у детей при воздействии химических факторов среды обитания // Гигиена и санитария, 2014. № 93 (2). С. 23-26. [Lanin D.V., Zaitseva N.V., Zemlyanova M.A., Dolgih O.V., Dianova D.G. Characteristics of regulatory system in children exposed to the environmental chemical factors. Gigiena i sanitariya = Hygiene and Sanitation, 2014, no. 93 (2), pp. 23-26. (In Russ.)]
  4. Современный Красноярский край [Электронный ресурс] // Красноярский край. Официальный портал. Режим доступа: http://www.krskstate.ru/ (дата обращения: 24.05.2020). [Modern Krasnoyarsk territory [Electronic resource]. Krasnoyarsk region. Official portal. Access mode: http://www.krskstate.ru/ (date of the application: 24.05.2020)].
  5. Старкова К.Г., Аликина И.Н., Гусельников М.А., Никоношина Н.А., Кривцов А.В., Перминова И.В. и др. Изменение иммунных регуляторных показателей у детского населения в условиях промышленного загрязнения металлами // Российский иммунологический журнал, 2017. № 11 (3). С. 512-514. [Starkova K.G., Alikina I.N., Guselnikov M.A., Nikonoshina N.A., Krivtsov A.V., Perminova I.V. et al. Changes of immune regulatory markers in children’s population under conditions of industrial pollution by metals. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology, 2017, no. 11 (3), pp. 512-514. (In Russ.)]
  6. MacGillivray D.M., Kollmann T.R. The role of environmental factors in modulating immune responses in early life. Front. Immunol., 2014, no. 5, pp. 1-12.

Copyright (c) 2020 Chelakova Y.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № 77 - 11525 от 04.01.2002.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies