State of hyperglycemic and immunomodulator-treated peripheral blood lymphocytes

Cover Page

Cite item

Abstract

A level of peripheral blood lymphocytes, their functional activity and particularly produced range of secreted cytokines underlie severity of autoimmune process in diabetes mellitus (DM) against islet β-cells and other body cells. Because cytokine production is coordinated by macrophages and may also depend on accessibility lymphocyte nucleic acids, we aimed at examining level of nucleic acids in blood lymphocytes as well as cytokine production in rats with alloxan-induced diabetes treated with immunomodulatory 3-aminophthalhydrazide (3-APH).
Type 1 DM was modeled in male WISTAR rats by intraperitoneally administered alloxan at a dose of 300 mg/kg followed by inoculating 3-APH (2 mg/kg, in total 20 intramuscular injections). It was found that modeled diabetes was accompanied by increased number of peripheral blood lymphocytes and their bone marrow precursors, coupled to higher DNA amount in blood lymphocytes and associated with increased IL-6 and TNFα production. Administration of 3-APH to diabetic rats contributed to decreased number of peripheral blood lymphocytes and related DNA level likely resulting in decreased level of pro-inflammatory cytokine IL-6 in the blood serum and contributing to corrected hyperglycemia.

About the authors

I. F. Gette

Institute of Immunology and Physiology, Ural Branch, Russian Academy of Sciences

Author for correspondence.
Email: i.goette@yandex.ru

Gette Irina F. - PhD (Biology), Senior Research Associate, Laboratory of Morphology and Biochemistry

620049, Yekaterinburg, Pervomayskaya str., 106

Phone: 7 (909) 019-53-22

Russian Federation

I. G. Danilova

Institute of Immunology and Physiology, Ural Branch, Russian Academy of Sciences

Email: fake@neicon.ru

PhD, MD (Biology), Associate Professor, Deputy Director

Yekaterinburg

Russian Federation

References

  1. Гетте И.Ф. Содержание гистоновых белков в лимфоцитах крыс с аллоксановым диабетом в условиях модулирования активности макрофагов // Вестник уральской медицинской академической науки, 2014, № 3 (49). С. 18-19. [Gette I.F. Content of histone proteins in lymphocytes of rats with alloxan diabetes in terms of macrophage activity modulating. Vestnik uralskoy meditsinskoy akademicheskoy nauki = Bulletin of the Ural Medical Academic Science, 2014, no. 3 (49), pp. 18-19. (In Russ.)]
  2. Ланкин В.З., Тихазе А.К., Коновалова Г.Г., Одинокова О.А., Дорощук Н.А., Чазова И.Е. Оксидативный и карбонильный стресс как факторы модификации белков и деструкции ДНК при сахарном диабете // Терапевтический архив, 2018, № 10. С. 46-50. [Lankin V.Z., Tikhaze A.K., Konovalova G.G., Odinokova O.A., Doroshchuk N.A., Chazova I.E. Oxidative and carbonyl stress as a factors of the modification of proteins and DNA destruction in diabetes. Terapevticheskiy arkhiv = Therapeutic Archive, 2018, Vol. 90, no. 10, pp. 46-50. (In Russ.)]
  3. Маркушева Л.И., Савина М.И., Решина В.М., Тогузов Р.Т. Ядерные белки хроматина в оценке эффективности лечения больных псориазом // Клиническая лабораторная диагностика, 2000. № 7. С. 18-20. [Markusheva L.I., Savina M.I., Reshina V.M., Toguzov R.T. Chromatin nuclear proteins in evaluation of the effectiveness of treatment of patients with psoriasis. Klinicheskaya laboratornaya diagnostika = Russian Clinical Laboratory Diagnostics, 2000, no. 7, pp. 18-20. (In Russ.)]
  4. Сычева Л.П. Биологическое значение, критерии определения и пределы варьирования полного спектра кариологических показателей при оценке цитогенетического статуса человека // Медицинская генетика, 2007. № 11. С. 3-11. [Sycheva L.P. Biological value, scoring criteria and limits of a variation of a full spectrum karyological indexes of exfoliated cells for estimation of human cytogenetic status. Meditsinskaya genetica = Scientific and Practical Journal Medical Genetics, 2007, no. 11, pp. 3-11. (In Russ.)]
  5. Danilova I.G., Bulavintceva T.S., Gette I.F., Medvedeva S.Y., Emelyanov V.V., Abidov M.T. Partial recovery from alloxan-induced diabetes by sodium phthalhydrazide in rats. Biomed. Pharmacother., 2017,Vol. 95, pp. 103-110.
  6. Ferreira R.C., Simons H.Z., Thompson W.S., Cutler A.J., Dopico X.C., Smyth D.J., Mashar M., Schuilenburg H., Walker N.M., Dunger D.B., Wallace C., Todd J.A., Wicker L.S., Pekalski M.L. IL-21 production by CD4+ effector T cells and frequency of circulating follicular helper T cells are increased in type 1 diabetes patients. Diabetologia, 2015, Vol. 58, no. 4, pp. 781-790.
  7. Jukić T., Abidov M., Ihan A. A tetrahydrophthalazine derivative ‘sodium nucleinate” exerts a potent suppressive effect upon LPS-stimulated mononuclear cells in vitro and in vivo. Collegium Antropologicum, 2011, Vol. 35, no. 4, pp. 1219-1223.
  8. Zargari Samani O., Mahmoodnia L., Izad M., Shirzad H., Jamshidian A., Ghatrehsamani M., Kheiri S., Sadeghian L., Soltani A., Sarmadi A., Kaohsiung J. Alteration in CD8(+) T cell subsets in enterovirus-infected patients: An alarming factor for type 1 diabetes mellitus. Med. Sci., 2018, Vol. 34, no. 5, pp. 274-280.

Copyright (c) 2020 Gette I.F., Danilova I.G.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № 77 - 11525 от 04.01.2002.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies