Examining effects of NS1 specific antibodies on sublethal influenza infection and secondary bacterial pneumonia in mice

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Abstract

Influenza is a highly contagious respiratory disease widespread throughout the world that causes disease in humans, birds and many mammalian species. Annually, around 20% of the global human gets sick with influenza so that more than 500,000 people die its various complications. Secondary bacterial pneumonia poses the peak threat during influenza infection, being most frequently caused by S. pneumoniae. Multiple studies in humans confirm the negative impact of influenza virus infection on subsequent outcome of bacterial pneumonia and provides insight into increased morbidity and mortality due to complicated influenza infection. In particular, the last 2009 influenza pandemic caused by H1N1 virus revealed that 25-56% cases of severe disease forms were associated with secondary pneumonia, among which 14-46% of them were fatal. Based on the aforementioned, it is of high priority to investigate a role of influenza virus proteins in developing of pathogen synergism in viral-bacterial pneumonia, particularly influenza virus non-structural protein NS1. The study objective was to examine effects of NS1-specific antibodies on course of influenza infection and secondary bacterial pneumonia in mice. For this, we used an experimental model of sublethal influenza infection followed by secondary Streptococcus pneumoniae bacterial pneumonia. Influenza A/Puerto Rico/8/34 (H1N1) virus and S. pneumoniae No. 3405 strain were used to simulate influenza infection. Rabbit serum containing antibodies against recombinant NS1 protein from A/Puerto Rico/8/34 virus and native rabbit serum (contain no specific antibodies) were used for vaccination. The study was carried out with female BALB/c mice, weighing 20-22 g. Protective activity of animal serum was assessed by using the three criteria: infection-related mortality, life expectancy and body weight change. The data obtained showed that passive transfer of antibodies specific to influenza virus NS1 protein did not lowered viral replication in sublethal murine model of influenza infection. Subsequent secondary bacterial pneumonia induced by S. pneumoniae revealed no protective effect of anti-NS1 protein antibodies assessed by measuring survival rate, lung viral and bacterial titers in treated vs. control mice.

About the authors

N. P. Kartashova

I. Mechnikov Research Institute for Vaccines and Sera

Author for correspondence.
Email: nadezdakartasova10571@gmail.com

Kartashova Nadezhda P. - Junior Research Associate, Laboratory of Experimental Virology, O.G. Anjaparidze Department of Virology

105064, Moscow, Malyi Kazennyi lane, 5a, bldg 9

Phone: 7 (917) 584-59-61

Russian Federation

I. A. Leneva

I. Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

PhD, MD (Biology), Head, Laboratory of Experimental Virology, O.G. Anjaparidze Department of Virology,

Moscow

Russian Federation

I. N. Falynskova

I. Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

Research Associate, Laboratory of Experimental Virology, O.G. Anjaparidze Department of Virology

Moscow

Russian Federation

A. V. Poddubikov

I. Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

PhD (Biology), Head, Laboratory of Microbiology of Opportunistic Bacteria, Department of Microbiology

Moscow

Russian Federation

References

  1. Карташова Н.П., Вартанова Н.О., Поддубиков А.В., Ленева И.А. Разработка экспериментальной вторичной бактериальной пневмонии, индуцированной S. pneumoniae после заражения пандемическим и лабораторным штаммами вируса гриппа H1N1 // Вестник «Биомедицина и социология», 2018. Т. 3, № 3. С. 15-17. [Kartashova N.P., Vartanova N.O., Poddubikov A.V., Leneva I.A. Development of experimental secondary bacterial pneumonia induced by S. pneumoniae after infection with pandemic and laboratory strains of the H1N1 influenza virus. Vestnik “Biomeditsina i sotsiologiya” = Bulletin “Biomedicine and Sociology”, 2018. Vol. 3, no. 3, pp. 15-17. (In Russ.)]
  2. Ленева И.А., Егоров А.Ю., Фалынскова И.Н., Махмудова Н.Р., Карташова Н.П., Глубокова Е.А., Вартанова Н.О., Поддубиков А. Индукция вторичной бактериальной пневмонии у мышей при заражении пандемическим и лабораторным штаммами вируса гриппа H1N1 // Журнал микробиологии, эпидемиологии и иммунобиологии, 2019. № 1. С. 68-74. [Leneva I.А., Egorov A.Yu., Falynskova I.N., Makhmudova N.R., Kartashova N., Glubokova E.A., Vartanova N.O., Poddubikov A.V. Induction of secondary bacterial pneumonia in mice upon infection with pandemic and laboratory strains of the H1N1 influenza virus. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Microbiology, Epidemiology and Immunobiology, 2019, no. 1, pp. 68-74. (In Russ.)]
  3. Egorov A. The problem of bacterial complications post respiratory viral infections. MIR J., 2018, Vol. 5, no. 1, pp. 12-21.

Copyright (c) 2020 Kartashova N.P., Leneva I.A., Falynskova I.N., Poddubikov A.V.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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