Effects of NOD1 and NOD2 agonists polymuramyl as well as NOD2 agonist glucosaminylmuramyldipeptide on phenotype of neutrophile granulocyte CD64-CD16+CD32+CD11b+, CD16+CD62L+CD63-, CD16+CD62L+CD63+ subsets

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Abstract

Antimicrobial activity of neutrophilic granulocytes (NG) is based on effective recognition and elimination of microbial pathogens, as well as on complex intracellular signal transduction pathways interconnecting these processes. NG dysfunction leads to emergence of atypical infectious and inflammatory diseases recalcitrant to standard interventions, which requires new vector platforms aimed at restoring normal NG functioning and overcoming antibiotic resistance. Moreover, we emphasize about special interest paid to the NOD1 and NOD2 agonist polymuramyl and NOD2 agonist glucosaminylmuramyldipeptide. Objective of the study was to compare effects triggered by NOD1 and NOD2 agonist polymuramyl and NOD2 agonist glucosaminylmuramyldipeptide on phenotype of neutrophilic granulocyte subsets CD64- CD16+CD32+CD11b+, CD16+CD62L+CD63-, CD16+CD62L+CD63+ in the in vitro system. 64 samples of peripheral blood (PC) collected from 8 apparently healthy children (4 boys and 4 girls) aged 3 to 9 years were examined by flow cytometry (FC 500, Beckman Coulter, USA) assessing NG surface receptors CD64, CD16, CD32, CD11b, CD62L, CD63 with MonAb (Beckman Coulter International S. A., France) by analyzing NG number (%) expressing receptors examined, density of receptor expression measured as mean fluorescence intensity (MFI). For this, there were assessed intact peripheral blood NG from apparently healthy children (comparison group) as well as those exposed to polymuramyl (PM) (at concentration of 10-6 g/l) or glucosaminylmuramyl-dipeptide (GMDP) (at concentration of 10-6 g/l) for 60 minutes at 37 °С temperature. Comparative analysis of surface receptor expression was performed on CD64-CD16+CD32+CD11b+, CD16+CD62L+CD63- and CD16+CD62L+CD63+ NG subsets that suggested about positive transformation of activation parameters in circulating NG exposed to NOD1 and NOD2 agonist polymuramyl as well as NOD2 agonist glucosaminylmuramyldipeptide. At the same time, similar effects of varying intensity were revealed manifested as increased count of NG subsets CD16+CD62L+CD63+ bearing increased level of CD16 and reduced CD62L expression, as well as differences uncovered as significantly increased expression of surface membrane molecules CD16 and CD11b in CD64-CD16+CD32+CD11b+ NG subset from apparently healthy children exposed to polymuramyl as well as increased surface CD32 expression after incubation with GMDP.

About the authors

I. V. Nesterova

Kuban State Medical University; Peoples’ Friendship University of Russia

Author for correspondence.
Email: inesterova1@yandex.ru

Nesterova Irina V. - PhD, MD (Medicine), Professor, Chief Research Associate, Department of Clinical and Experimental Immunology and Molecular Biology, Central Research Laboratory; Professor, Department of Allergology and Immunology

117513, Moscow, Leninsky ave, 123, bldg 1, apt 593

Phone: 7 (916) 187-73-41

Russian Federation

G. A. Chudilova

Kuban State Medical University

Email: fake@neicon.ru

PhD (Biology), Associate Professor, Head, Department of Clinical and Experimental Immunology and Molecular Biology, Central Research Laboratory

Krasnodar

Russian Federation

V. N. Pavlenko

Kuban State Medical University

Email: fake@neicon.ru

Postgraduate Student, Department of Clinical Immunology, Allergology and Laboratory Diagnostics

Krasnodar

Russian Federation

S. V. Kovaleva

Kuban State Medical University

Email: fake@neicon.ru

PhD (Medicine), Associate Professor, Senior Research Associate, Department of Clinical Experimental Immunology and Molecular Biology

Krasnodar

Russian Federation

V. A. Tarakanov

Kuban State Medical University

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head, Department of Surgical Diseases of Childhood

Krasnodar

Russian Federation

N. K. Barova

Kuban State Medical University

Email: fake@neicon.ru

PhD (Medicine), Assistant Professor, Department of Surgical Diseases of Childhood

Krasnodar

Russian Federation

References

  1. Нестерова И.В., Колесникова Н.В., Чудилова Г.А., Ломтатидзе Л.В., Ковалева С.В., Евглевский А.А., Нгуен Т.З.Л. Новый взгляд на нейтрофильные гранулоциты: переосмысление старых догм (часть 2) // Инфекция и иммунитет, 2018. Т. 8, № 1. С. 7-18. [Nesterova I.V., Kolesnikova N.V., Chudilova G.A., Lomtatidze L.V., Kovaleva S.V., Evlevsky A.A., Nguyen T.Z.L. A new look at neutrophilic granulocytes: rethinking old dogmas (Part 2). Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2018, Vol. 8, no. 1, рр. 7-18. (In Russ.)] doi: 10.15789/2220-7619-2018-1-7-18.
  2. Alyami H.M., Finoti L.S., Teixeira H.S., Aljefri A., Kinane D.F., Benakanakere M.R. Role of NOD1/NOD2 receptors in Fusobacterium nucleatum mediated NETosis. Microb. Pathog., 2019, Vol. 131, pp. 53-64.
  3. Chamaillard M., Hashimoto M., Horie Y., Masumoto J., Qiu S., Saab L., OguraY., Kawasaki A., Fukase K., Kusumoto Sh., Valvano M.A., Foster S.J., Mak T.W., Nuñez G., Inohara N. An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid. Nat. Immunol., 2003, Vol. 4, no. 7, pp. 702-707.
  4. Dagil Y.A., Arbatsky N.P., Pashenkov M.V., Alkhazova B.I., L’vov V.L., Mazurov D.V. The dual NOD1/ NOD2 Agonism of muropeptides containing a meso-diaminopimelic acid residue. PLoS ONE, 2016, Vol. 11, no. 8, e0160784. doi: 10.1371/journal.pone.0160784.
  5. Ekman A.-K., Cardell L.O. The expression and function of Nod-like receptors in neutrophils. Immunology, 2010, Vol. 130, no. 1, pp. 55-63.
  6. Girardin S.E., Boneca I.G., Viala J., Chamaillard M., Labigne A., Thomas G., Philpott D.J., Sansonetti P.J. Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J. Biol. Chem., 2003, Vol. 278, no. 11, pp. 8869-8872.
  7. Mastej K., Adamiec R. Neutrophil surface expression of CD11b and CD62L in diabetic microangiopathy. Acta Diabetol., 2008, Vol. 45, no. 3, pp. 183-190.
  8. Meshcheryakova E., Makarov E., Philpott D., Andronova T., Ivanov V. Evidence for correlation between the intensities of adjuvant effects and NOD2 activation by monomeric, dimeric and lipophylic derivatives of N-acetylglucosaminy N-acetylmuramyl peptides. Vaccine, 2007, Vol. 25, no. 23, pp. 4515-4520.
  9. Metelitsa L.S., Gillies S.D., Super M., Shimada H., Reynolds C.P., Seeger R.C. Antidisialoganglioside/ granulocyte macrophage-colony-stimulating factor fusion protein facilitates neutrophil antibody-dependent cellular cytotoxicity and depends on FcgammaRII (CD32) and Mac-1 (CD11b/CD18) for enhanced effector cell adhesion and azurophil granule exocytosis. Blood, 2002, Vol. 99, no. 11, pp. 4166-4173.
  10. Moreira L.O., Zamboni D.S. NOD1 and NOD2 signaling in infection and inflammation. Front. Immunol., 2012, Vol. 3, 328. doi: 10.3389/fimmu.2012.00328.

Copyright (c) 2020 Nesterova I.V., Chudilova G.A., Pavlenko V.N., Kovaleva S.V., Tarakanov V.A., Barova N.K.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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