BASOPHIL ACTIVATION TEST IN THE DIAGNOSIS OF CHRONIC URTICARIA


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Abstract

Chronic urticaria (CU) is a serious medical and social problem that significantly reduces the quality of patients life. Chronic autoimmune urticaria (CAU) is characterized by the presence of autoantibodies to IgE or its high affinity FcεRI receptor. The study included 16 patients with CU. The autologous serum skin test (ASST) was positive in 31% of patients. The level of elevated total IgE was detected in 38% of patients. A higher number of basophils with activation markers CD203c (14.0 ± 2.7% vs 9.3 ± 2.3%) and increased values of the stimulation index (SI) (2.0 ± 0.4 vs 1.3 ± 0, 3) were identified in patients with positive ASST compared to patients with negative ASST. The results of intracutaneous testing and positive correlation with expression of basophil activation markers made it possible to diagnose CAU in 64% of cases. Basophil activation test in vitro is a promising method for the diagnosis of CAU, which allows to determine further therapeutic tactic.

About the authors

Y. I. Kozlova

North-Western State Medical University named after I. I. Mechnikov

Author for correspondence.
Email: kozlova510@mail.ru

PhD, Associate professor of the Department of Clinical Mycology, Allergology and Immunology,

Saint-Petersburg

Russian Federation

E. V. Burygina

North-Western State Medical University named after I. I. Mechnikov

Email: fake@neicon.ru

MD, Senior Lab Assistant of the Department of Clinical Mycology, Allergology and Immunology,

Saint-Petersburg

Russian Federation

A. V. Sobolev

North-Western State Medical University named after I. I. Mechnikov

Email: fake@neicon.ru

PhD, Professor of the Department of Clinical Mycology, Allergology and Immunology, Head Allergologist of the North-Western Federal District and Leningrad Oblast, 

Saint-Petersburg

Russian Federation

N. N. Klimko

North-Western State Medical University named after I. I. Mechnikov

Email: fake@neicon.ru

PhD, Professor, Head Professor of the Department of Clinical Mycology, Allergology and Immunology,

Saint-Petersburg

Russian Federation

References

  1. Zuberbier T., Aberer W., Asero R., et al. The EAACI/ GA2LEN/EDF/WAO guideline for the definition, classification, diagnosis, and management of urticaria. Allergy, 2018; 73(7): 1393–1414.
  2. Kaplan A. P. Diagnosis, pathogenesis, and treatment of chronic spontaneous urticaria. Allergy Asthma Proc. 2018 May 1; 39(3): 184–190.
  3. Kim Z., Choi B. S., Kim J. K., Won D. I. Basophil markers for identification and activation in the indirect basophil activation test by flow cytometry for diagnosis of autoimmune urticaria. Ann Lab Med. 2016 Jan; 36(1): 28–35.
  4. Curto-Barredo L., Yelamos J., Gimeno R., Mojal S., Pujol Ramon M., Giménez-Arnau A. Basophil activation test identifies the patients with chronic spontaneous Urticaria suffering the most active disease. Immun Inflamm Dis. 2016; 4(4): 441–445.

Copyright (c) 2019 Kozlova Y.I., Burygina E.V., Sobolev A.V., Klimko N.N.

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