Features of altered immune status in acute brain concussion

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Abstract

Traumatic brain injury (TBI) is one of the most common type of injuries, so that its mild form prevails in overall injury pattern. Currently, it is known that brain injury triggers immune system response, but its role in translating into clinical manifestations, potential complications and sequelae remains poorly understood. It necessitates assessment of cellular immunity in patients with acute TBI of varying severity followed by investigating relationship between identified changes. It is now believed that immune system plays a lead role in brain functioning. It may be accounted for by interplay between peripheral immune cells and the brain, which may become augmented during developing immune response.
Here we quantitatively assessed composition of major peripheral blood helper T cell subsets in TBI patients by flow cytometry measuring percentage of central (CM, CD45RA-CD62L+) and effector (EM, CD45RA-CD62L-) memory Th cells. It was found that percentage of Th17 (CXCR5-CXCR3-CCR6+CCR4-), DP Th17 (CXCR5-CXCR3+CCR6+CCR4+) within CD3+CD4+T cell population were significantly increased (p < 0.05) compared to control group. Moreover, percentage of Th1/Th17 subset (CXCR5-CXCR3+CCR6+CCR4-) was significantly increased (p < 0.05) within EM and CM T cell subsets compared to control group. In addition, percentage of Th1 (CXCR5-CXCR3+CCR6-CCR4) was also significantly elevated in CD3+CD4+, EM and CM T cells compared to apparently healthy subjects. Hence, the data obtained allow to consider immune reactions among crucial arms in TBI pathogenesis related to concussion and its consequences. Thus, brain concussion affects cellular immune response triggering distortion in CD3+CD4+T cell composition as well as percentage of helper central and effector memory T cells.
Hence, the changes revealed in patients with acute brain concussion may predetermine disease course and developing long-term complications, which requires advancing therapeutic and rehabilitation protocols in such patients.

About the authors

A. O. Norka

St. Petersburg State Pediatric Medical University; First St. Petersburg State I. Pavlov Medical University

Author for correspondence.
Email: norka-anna@mail.ru

Norka Anna O. - Senior Laboratory Assistant, Department of Immunology; Resident, Department of Neonatology with courses of neurology and obstetrics-gynecology at FP and
DPO

197022, St. Petersburg, L. Tolstoy str., 6-8

Phone: 7 (911) 218-85-00

Russian Federation

S. V. Vorobyev

St. Petersburg State Pediatric Medical University; V. Almazov National Medical Research Centre

Email: fake@neicon.ru

PhD, MD (Medicine), Chief Research Associate, Research Laboratory of Neurology and Neurorehabilitation; Professor, Department of Clinical Laboratory Diagnostics

St. Petersburg

Russian Federation

R. N. Kuznetsova

First St. Petersburg State I. Pavlov Medical University; St. Petersburg Pasteur Institute

Email: fake@neicon.ru

PhD (Medicine), Associate Professor, Department of Immunology; Allergist-Immunologist, Medical Centre

St. Petersburg

Russian Federation

M. K. Serebriakova

Institute of Experimental Medicine

Email: fake@neicon.ru

Graduate Student, Research Associate, Department of Immunology

St. Petersburg

Russian Federation

I. V. Kudryavtsev

Institute of Experimental Medicine; First St. Petersburg State I. Pavlov Medical University

Email: fake@neicon.ru

PhD (Biology), Senior Research Associate, Department of Immunology, Institute of Experimental Medicine; Associate Professor, Department of Immunology

St. Petersburg

Russian Federation

S. N. Kovalenko

S. Kirov Military Medical Academy

Email: fake@neicon.ru

Lecturer, Department of Neurosurgery

St. Petersburg

Russian Federation

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Copyright (c) 2020 Norka A.O., Vorobyev S.V., Kuznetsova R.N., Serebriakova M.K., Kudryavtsev I.V., Kovalenko S.N.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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