Role of MP-4 myelopeptide in regulation of antibody production and functional activity of macrophages under stress conditions

Cover Page

Cite item


Myelopeptides are endogenous low-molecular weight peptides with immunoregulatory activity. Each of these myelopeptides has an individual sequence and plays a role in correction of immune system disorders. In our earlier studies, we have shown immunomodulatory effect of MP-3, MP-5, MP-6 myelopeptides upon functioning of peritoneal macrophages under the conditions of immobilization and cold stresses. The aim of this work was to investigate the effect of MP-4 myelopeptide upon antibody formation, production of reactive oxygen species, and absorptive activity of peritoneal macrophages in mice under the immobilization stress. Materials and methods. The in vivo experiments were carried out in 24 male Swiss mice weighing 17-22 g. Two-or six-hour immobilization stress was produced in the animals. MP-4 was injected intraperitoneally 30 min before the onset of stress, at a dose of 40 μg/kg. The animals were divided into 4 groups: 1, control; 2, stress; 3, stress + MP-4; 4, MP-4 injection. On day +5, cellularity and the number of antibody-forming cells (AOC) in spleen were assessed by the method of local hemolysis in an agarose gel according to Jerne. Production of reactive oxygen species by peritoneal cells was assessed using the luminol-dependent chemiluminescence (LZHL) reaction. The absorption capacity of peritoneal macrophages was assessed using a standard method using a BDFACSCalibur laser flow cytometer. Labeled St. aureus at a final concentration of 108 cells/ml were used as phagocytosis targets. Statistical analysis was performed using the unpaired Student's t-test. Results. It was found that two-hour immobilization stress did not affect the absorption activity of peritoneal leukocytes, whereas six-hour stress suppressed phagocytosis. Injection of MP-4 in stress-exposed animals did not lead to changes in phagocytic activity of peritoneal macrophages. MP-4 did not correct the stress-induced decrease in the number of antibody-forming cells from the spleen during six hours of immobilization, nor stress-induced increase in absolute amounts of AOC under the two-hour immobilization stress. In the group of animals receiving MP-4 and immobilized for two-hours, an increase in the number of nucleated cells was increased, as compared with the control group. Thus, in contrast to the previously studied myelopeptides, MP-4 did not show pronounced immunomodulatory effects upon these parameters.

About the authors

T. V. Gavrilova

E. Wagner Perm State Medical University; Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm Federal Research Center, Ural Branch, Russian Academy of Sciences

Author for correspondence.

Tatyana V. Gavrilova - PhD, MD (Medicine), Professor, Head, Department of Ophthalmology, Perm State National Research University; Leading Research Associate, Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences.

614000, Perm, Petropavlovskaya str., 26.

Phone: 7 (902) 471-62-08.

Russian Federation

D. A. Oralova

Perm State National Research University


Student, Faculty of Biology, Perm State National Research University.


Russian Federation

O. N. Gein

Perm State Pharmaceutical Academy


PhD (Biology), Associate Professor, Department of Pharmacology, Perm State Pharmaceutical Academy.


Russian Federation

M. V. Chereshneva

Institute of Immunology and Physiology, Ural Branch, Russian Academy of Sciences


PhD, MD (Medicine), Professor, Main Research Associate, Institute of Immunology and Physiology, Ural Branch, Russian Academy of Sciences.


Russian Federation


  1. Гаврилова Т.В., Гейн С.В. Иммунорегугулирующие эффекты миелопептидов при экспериментальном проникающем ранении глаза. Екатеринбург: УрОРАН, 2004. 102 с.
  2. Гаврилова Т.В., Гейн О.Н., Гейн С.В., Черешнева М.В., Черешнев В.А. Миелопептиды в регуляции функциональной активности макрофагов при стрессе in vivo // Вестник Пермского федерального исследовательского центра, 2020. № 1. С. 53-60.
  3. Гейн С.В., Гаврилова Т.В., Журавлёва Л.С., Черешнева М.В., Черешнев В.А., Кирилина Е.А. Влияние МП-3 на динамику респироторного взрыва и продукцию IL-1 в и TNF-a перитонеальными макрофагами мыши при стрессе in vivo. Доклады Академии наук, 2014. Т. 455, № 2. С. 232-234.
  4. Петров Р.В., Михайлова А.А., Фонина Л.А., Степаненко Р.Н. Миелопептиды. М.: Наука, 2000. 181 с.
  5. Фонина Л.А., Трещалина Е.М., Белевская Р.Г., Азьмуко А.А., Ефремов М.А., Седакова Л.А., Кирилина Е.А. Синтез и противоопухолевые свойства миелопептида МП-1. Биоорганическая химия, 2012. Т. 38, № 4. С. 406-412.
  6. Jerne N.K., Nordin A.A. Plaque Formation in Agar by Single Antibody-Producing Cells. Science, 1963, Vol. 140, no. 3365, 405. doi: 10.1126/science.140.3565.405.
  7. Mikhailova A., Fonina L., Kirilina E., Shanurin S., Gur'yanov S., Malakhov A., Nesmeyanov V., Petrov R. Immunoregulatory properties of hexapeptide isolated from porcine bone marrow cell culture. Regul. Pept., 1994, Vol. 53, Iss. 3, pp. 203-209.
  8. Marinova Z., Vukojevic V., Surcheva S., Yakovleva T., Cebers G., Pasikova N., Usynin I., Hugonin L., Fang W., Hallberg M., Hirschberg D., Bergman T., Langel U., Hauser K.F., Pramanik A., Aldrich J.V., Graslund A., Terenius L., Bakalkin G. Translocation of dynorphin neuropeptides across the plasma membrane. A putative mechanism of signal transmission. J. Biol Chem., 2005, Vol. 280, no. 28, pp. 26360-26370.

Supplementary files

There are no supplementary files to display.

Copyright (c) 2021 Gavrilova T.V., Oralova D.A., Gein O.N., Chereshneva M.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № 77 - 11525 от 04.01.2002.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies