Experimental evaluation of cytoprotective effect of probiotic metabolites and selection criteria of Bacillus Subtilis strains for development of new immunotropic biohepatoprotector

Cover Page

Cite item


The present work concerns a study of cytoprotective effect of biologically active substances (BAS) produced by probiotic microorganisms from the Bacillus genus. These substances were obtained from a sterile fugate of probiotic Bacillus subtilis VKPM B-3679 culture (a biosporicin-producing strain) and added to the culture of isolated hepatocytes, when modeling their in vitro toxic damage. Sterile buffered culture of Bacillus subtilis B-3679 was prepared by sterilizing filtration of the culture supernatant of this strain. The work was carried out using a passageable L-41 cell culture strain, which allows to assess toxicity of various substrates upon cell cultures and protection against toxic effects at different concentrations. At the first stage of study, the maximal non-toxic dose of sterile fugate and the minimal toxic dose of CCl4 established for the cultured L-41 cells. At the next stage, cytoprotective effect of BAS originating from the of B. subtilis B-3679 strain fugate, was studied and tested with L-41 cell line. Cytoprotective and regenerative effects of BAS containing in the fugate of B. subtilis B-3679 strain were demonstrated in the model of toxic damage using the culture of isolated hepatocytes. In preclinical studies, to assess the toxicity and safety of the experimental sample of a new biohepatoprotector for experimental animals, we have found that the complex of biologically active substances (metabolites) is non-toxic and safe for laboratory animals when administered intragastrically and intraperitoneally, and it does not cause any pathological changes in their internal organs and tissues. The basis of the new biohepatoprotection effect, which may provide a multifunctional action, allowing to effectively restore depressed liver functions, with simultaneous normalization of immunological parameters, is its active biocomponent, i.e., metabolites of probiotic spore-forming bacteria, which, when if brought to the body, produce a complex of biologically active metabolites (antibiotics, proteolytic, amylolytic, and other enzymes, immunoglobulins, as well as interleukins, vitamins, proteins, amino acids, and others bioactive substances). Due to experimentally established cytoprotective effect of the complex of BAS, the components of fugate culture of B. subtilis strain B-3679, will allow us to develop new promising medical immunobiological drugs that may provide a protective effect on human organs and tissues. As a result it was found that BAS, as components of fugate from B. subtilis B-3679 strain show both a pronounced cytoprotective effect, and a positive action upon regenerative abilities of liver cells, which is a significant factor for the future use of this strain as a biocomponent of a new immunotropic biohepatoprotector.

About the authors

N. A. Zabokritskiy

Institute of Immunology and Physiology, Ural Branch, Russian Academy of Sciences

Author for correspondence.
Email: pharmusma@rambler.ru

Nikolai A. Zabokritskiy - PhD, MD (Medicine), Associate Professor, 5enior Research Associate, Laboratory of Immunophysiology and Immunopharmacology, Institute of Immunology and Physiology, Ural Branch, Russian Academy of 5ciences, Yekaterinburg, Russian Federation.

620049, Yekaterinburg, Pervomaiskaya str., 106.

Phone: 7 (922) 110-11-14.

Russian Federation


  1. Ардатская М.Д., Столярова Л.Г., Архипова Е.В., Филимонова О.Ю. Метабиотики как естественное развитие пробиотической концепции // Рецепт, 2019. Т. 2, № 22. С. 291-298.
  2. Забокрицкий Н.А. Оценка иммунотропного действия пробиотика бацилакт в составе трансдермальных терапевтических систем // Российский иммунологический журнал, 2017. Т. 11, № 2. С. 126-129.
  3. Забокрицкий Н.А. Принципиальные направления научных исследований по обоснованию и разработке новых иммунобиологических препаратов // Экспериментальная и клиническая фармакология, 2018. Т. 81, № S. С. 85-86.
  4. Забокрицкий Н.А., Сарапульцев П.А. Экспериментальное обоснование возможности создания нового метаболического препарата // Российский иммунологический журнал, 2018. Т. 12, № 3. С. 295-300.
  5. Забокрицкий Н.А. Фармакологическая оценка иммунотропной активности нового гелевого метабиотика на факторы клеточного и гуморального иммунитета при экспериментальном моделировании термических ожогов кожи // Российский иммунологический журнал, 2020. Т. 23, № 2. С. 125-132.
  6. Лабинская А.С., Блинкова Л.П., Ещина А.С., Булаева Г.В., Вертиев Ю.В., Винокуров А.Е., Горобец О.Б., Дарбеева О.С., Жиленков Е.Л., Зверьков Д.А., Иванова С.М., Иванова Т.С., Корн М.Я., Кривопалова Н.С., Лукин И.Н., Мельникова В.А., Нехорошева А.Г., Романова Ю.М., Сидоренко С.В., Скаженик В.Ю., Скала Л.З., Трухина Г.М. Общая и санитарная микробиология с техникой микробиологических исследований. - СПб.: Лань, 2016. 588 с.
  7. Lee N.K., Paik H.D., Kim W.S. Bacillus strains as human probiotics: characterization, safety, microbiome, and probiotic carrier. Food Sci. Biotechnol., 2019, Vol. 28, no. 5, pp. 1297-1305.

Supplementary files

There are no supplementary files to display.

Copyright (c) 2021 Zabokritskiy N.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № 77 - 11525 от 04.01.2002.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies