Influence of glycodelin on morphofunctional state of secondary immune organs in experimental model of allogeneic transplantation

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Abstract

Glycodelin (PP14, PAEP, alpha-2-microglobulin, dimeric glycoprotein with a molecular weight of 42 to 56 kDa) is a marker of reproductive tissue receptivity. Immunoregulatory potential of glycodelin allows us to consider it among key factors that promote maternal immune tolerance to the developing embryo. In general, glycodelin has prospects for use in biomedicine as a biopharmaceutical to treat post-transplant complications. This work aimed to study the effect of glycodelin on morpho-functional state of the secondary organs of immune response in the course of in vivo experiment during allogeneic transplantation of bone marrow cell suspension to Wistar rats. The experiment was performed in white male Wistar rats aged 2-3 months (body mass, 250 g). The animals were kept at the Perm State National Research University animal clinic under GOST 33216-2014 “Rules for working with laboratory rodents and rabbits”. The animals were sacrificed on the 3rd, 7th, 14th, and 21st days according to international rules for working with experimental animals. In the control group, animals (n = 6) were injected once with a suspension of camptothecin-treated bone marrow cells; in experimental group, the animals (n = 12) were injected with a bone marrow cells in combination with glycodelin administered 4 times.

We used recombinant glycodelin (MyBioSource, Germany), which was administered to the animals at a concentration corresponding to pregnancy state (0.75 μg/mL). Intraperitoneal injection of bone marrow cells simultaneously with glycodelin over the terms of experiment (21 days) showed that allogeneic transplantation caused hyperplasia of spleen and lymph nodes. In the functional zones of organs, proliferation and differentiation of immune cells developed. Injection of glycodelin at the early stages of experiment (3-7 days) caused an increase in proliferative processes in the organs of both T- and B-dependent immunity. Moreover, there were no signs of inflammation and apoptotic cell death in the organs. Since the 14th day of the experiment, eosinophilic infiltration of the organs was evident, being an indirect positive sign of response to the transplanted cells. By the end of study, differentiation processes dominated over proliferation in the organs. Thus, glycodelin stabilizes proliferative processes and promotes emergence of a new cell generation, thus supporting host response to the transplant. Apparently, glycodelin can participate in development of adaptive mechanism in secondary organs of immune system.

About the authors

N. P. Loginova

E. Wagner Perm State Medical University

Email: natalitsa@yandex.ru

PhD, MD (Medicine), Associate Professor, Head, Department of Histology, Embryology and Cytology, E. Wagner Perm State Medical University.

Perm.

Russian Federation

Ya. N. Troynich

E. Wagner Perm State Medical University

Email: yananiktroynich@mail.ru

Lecturer, Department of Histology, Embryology and Cytology, E. Wagner Perm State Medical University.

Perm.

Russian Federation

Yu. A. Charushina

E. Wagner Perm State Medical University

Email: julia_pochta@mail.ru

Lecturer, Department of Histology, Embryology and Cytology, E. Wagner Perm State Medical University.

Perm.

Russian Federation

S. A. Zamorina

Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm Federal Research Center, Ural Branch, Russian Academy of Sciences; Perm State National Research University

Author for correspondence.
Email: mantissa7@mail.ru

Svetlana A. Zamorina - PhD, MD (Biology), Leading Research Associate, Laboratory of Ecological Immunology, Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Professor, Microbiology and Immunology Department, Perm State National Research University.

614081, Perm, Golev str., 13.

Phone: 7 (342) 280-77-94.

Russian Federation

References

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  2. Посисеева Л.В., Герасимов А.М., Петрова У.Л. Гликоделин в акушерско-гинекологической практике: прошлое, настоящее, будущее // Проблемы репродукции, 2020, Т. 26, № 3. С. 11-22.
  3. Dixit A., Balakrishnan B., Karande A.A. Immunomodulatory activity of glycodelin: implications in allograft rejection. Clin. Exp. Immunol., 2018, Vol. 192, no. 2, pp. 213-223.
  4. Schneider M., Meister M., Muley T. Glycodelin as diagnostic and prognostic marker and for monitoring treatment of lung diseases. European patent application, Bulletin 2016.06. EP2982765A1.
  5. Schneider M.A., Granzow M., Warth A., Schnabel P.A., Thomas M., Herth F.J., Dienemann H., Muley T., Meister M. Glycodelin A new biomarker with immunomodulatory functions in non-small cell lung cancer. Clin. Cancer Res., 2015, Vol. 21, no. 15, pp. 3529-3540.

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Copyright (c) 2021 Loginova N.P., Troynich Y.N., Charushina Y.A., Zamorina S.A.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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