Features of polymorphic site combinations of Toll-like receptor (TLR) genes in children with ventricular septal defects

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Abstract

Congenital heart disease is the leading cause of the childhood disability and mortality. The development of new technologies has made it possible to advance in diagnosis and treatment of congenital heart disease, but its etiology is poorly understood. Considering multifactorial origin of this disease, a search for biomarkers having fundamental and practical importance is of current interest. Study of the genetic component is one of the areas of research. E.g., Toll-like receptors (TLR) are important regulators of susceptibility to infectious and non-infectious diseases. Polymorphic variants with single nucleotide substitutions in these genes may be accompanied by changes in the structure and expression of the encoded proteins, thus affecting functional activity of these receptors. Our aim was to study some genetic predictors of congenital heart defects in children. Materials and methods. We examined 47 children (21 girls and 26 boys) with congenital heart defects: 23 children had a ventricular septal defect (VSD); 24 children, atrial septal defect (ASD). The patients in the main group were 1 to 9 years old. As a control group, 96 conditionally healthy age- and sex-matched children were examined. Real-time genotyping was carried out with Viia7 real-time PCR system (Applied Biosystems, USA) using TaqMan probes of four TLR genes (TLR1 rs5743611, rs5743551, TLR2 rs5743708, rs3804099, TLR4 rs4986791, rs4986790, TLR6 rs3775073, rs5743810). Results. The distribution of all studied polymorphic variants corresponded to the HardyWeinberg equilibrium. It was revealed that sporadic nonsydromal VSD are associated with G allele of the TLR2 gene (rs5743708) and T allele of the TLR6 gene (rs3775073). According to ROC analysis, the combination of these alleles is a significant parameter showing high degree of sensitivity (82.4%). Both TLR2 (rs5743708) and TLR6 (rs3775073) gene variants define amino acid substitutions (missense mutations) in TLR molecules. Thus, we concluded that genetic testing can be used to identify risk groups at the early stages.

About the authors

A. V. Shabaldin

Research Institute for Complex Issues of Cardiovascular Diseases

Author for correspondence.
Email: weit2007@yandex.ru
ORCID iD: 0000-0002-8785-7896

Shabaldin Andrey V. - PhD, MD (Medicine), Associate Professor, Leading Research Associate, Laboratory of Heart Defects

650002, Kemerovo, Sosnoviy blvrd, 6

Russian Federation

A. V. Tsepokina

Research Institute for Complex Issues of Cardiovascular Diseases

Email: cepoav1991@gmail.com
ORCID iD: 0000-0002-4467-8732

Junior Research Associate, Laboratory for Genomic Medicine

650002, Kemerovo, Sosnoviy blvrd, 6

Russian Federation

S. A. Shmulevich

Research Institute for Complex Issues of Cardiovascular Diseases

Email: shmulsa@kemcardio.ru
ORCID iD: 0000-0002-7316-2962

PhD (Medicine), Senior Lecturer, Scientific and Educational Department

650002, Kemerovo, Sosnoviy blvrd, 6

Russian Federation

A. V. Ponasenko

Research Institute for Complex Issues of Cardiovascular Diseases

Email: ponaav@kemcardio.ru
ORCID iD: 0000-0002-3002-2863

PhD (Medicine), Head, Laboratory of Genomic Medicine

650002, Kemerovo, Sosnoviy blvrd, 6

Russian Federation

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Copyright (c) 2021 Shabaldin A.V., Tsepokina A.V., Shmulevich S.A., Ponasenko A.V.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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