Anticytokine activity and the ability to produce cytokine-like substances of staphylococci isolated from the prostatic secretions in men with symptoms of urogenital infection

Cover Page

Cite item

Abstract

To be causal agents for infectious and inflammatory diseases, the microorganisms should have a pathogenic potential, the framework of which is based on persistence factors that ensure their protection from various effectors of the host's immunity. There is evidence that some microorganisms can have anti-cytokine activity (ACA), and at the same time, many microorganisms many synthesize cytokine-like substances, thus, in fact, exhibit cytokine activity. The aim of the present study was to establish the presence of cytokine-like substances and anti-cytokine activity in Staphylococcus strains isolated from prostate secretions in men with symptoms of urogenital infection. This study involved 24 clinical isolates of different Staphylococci types isolated from the prostate gland secretions in men with symptoms of urogenital infection. Ability of staphylococci to cause changes in the IL-4, IL-6, IL-8, IL-10 and TNFα contents was carried out by co-incubating bacteria suspended in normal saline solution together with cytokine solutions, at a ratio of 1:1 (experimental samples). As a control, a solution of cytokines supplemented by normal saline was used. Concentration of cytokines in experimental and control samples was determined by enzyme immunoassay. Anti-cytokine activity (ACA) was expressed as a percentage (%) of cytokine inactivation in experimental mixtures compared to the controls, cytokine activity, as % of cytokine production in the experimental samples compared to the control.

The studied staphylococci showed anti-cytokine activity against IL-4 and IL-10, and exhibited ability to produce IL-6 and TNF. At the same time, among staphylococci, there were strains both showing anti-cytokine activity against IL-8, and producing this cytokine.

Among different staphylococcal species isolated from prostate secretions in men with symptoms of urogenital infection, the presence of anti-cytokine activity and the ability to produce cytokines was detected; their production (presence and level) was characterized by intraspecific variability. S. xylosus proved to be the most active cytokine producer, which also have the highest anti-cytokine activity against IL-4.

About the authors

O. L. Kartashova

Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences

Author for correspondence.
Email: labpersist@mail.ru

Olga L. Kartashova - PhD, MD (Biology), Associate Professor, Leading Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms, Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences.

460000, Orenburg, Pionerskaya str., 11.

Phone: 7 (353) 277-44-63.

Russian Federation

T. M. Pashkova

Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences

Email: labpersist@mail.ru

PhD, MD (Biology), Leading Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms, Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences.

Orenburg.

Russian Federation

O. A. Pashinina

Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences

Email: labpersist@mail.ru

PhD (Biology), Senior Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms, Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences.

Orenburg.

Russian Federation

N. V. Morozova

Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences

Email: labpersist@mail.ru

PhD (Biology), Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms, Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences.

Orenburg.

Russian Federation

References

  1. Bukharin O.V. Persistence of pathogenic bacteria]. Moscow: Meditsina; Ekaterinburg: Ural Branch of the Russian Academy of Sciences, 1999. 366 p.
  2. Bukharin O.V., Ivanova E.V., Perunova N.B., Chainikova I.N., Nikiforov I.A., Bondarenko T.A. Immunoregulatory properties of metabolites of bifidobacteria in eubiosis and dysbiosis of the human large intestine. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Microbiology, Epidemiology and Immunobiology, 2015, no. 4, pp. 89-96. (In Russ.)
  3. Bukharin O.V., Perunova N.B., Chainikova I.N., Ivanova E.V., Smolyagin A.I. Anticytokine activity of microorganisms. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Microbiology, Epidemiology and Immunobiology, 2011, no. 4, pp. 56-61. (In Russ.)
  4. Zurochka A.V., Dukardt V.V., Zurochka V.A., Dobrynina M.A., Zueva E.B., Tyapaeva Ya.V., Gritsenko V.A. Bacteria as producers of cytokine-like substances. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology, 2017, Vol. 11, no. 3, pp. 374-376. (In Russ.)
  5. Zurochka V.A., Zurochka A.V., Dukart V.V., Zueva E.B., Dobrynina M.A., Tyapaeva Ya.V., Gritsenko V.A. Assessment of the levels of cytokine-like substances in the supernatants of bacterial cultures. Meditsinskaya immunologiya = Medical immunology (Russia), 2017, Vol. 19, no. S, p. 45.(In Russ.)
  6. Lakin G.F. Biometrics. Moscow: Higher School, 1990. 352 p.
  7. Sycheva M.V., Pashkova T.M., Kartashova O.L., Pashinina O.A., Popova L.P. Characterization of the anticytokine activity of enterococci. Byulleten Orenburgskogo nauchnogo tsentra UrO RAN = Bulletin of the Orenburg Scientific Center of the Ural Branch of the Russian Academy of Sciences, 2015, Vol. 3, pp. 1-6. [Electronic resource]. Access mode: http://elmag.uran.ru:9673/magazine/Numbers/2015-3/Articles/SMV-2015-3.pdf.
  8. Fomina L.O., Fayzullina A.I., Zurochka V.A., Dobrynina M.A., Symbircev A.S., Gritsenko V.A. Comparative evaluation cytokine-like activity of Staphylococcus aureus by methods multiplex and ELISA. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology, 2018, Vol. 12, no. 3, pp. 460-465. (In Russ.)

Supplementary files

There are no supplementary files to display.


Copyright (c) 2021 Kartashova O.L., Pashkova T.M., Pashinina O.A., Morozova N.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies