PHENOTYPIC PROFILE SUBSET CD64–CD16⁺CD32⁺CD11b⁺, CD64⁺CD16⁺CD32⁺CD11b⁺ NEUTROPHIL GRANULOCYTES IN HEALTHY NEWBORNS, CONDITIONALLY HEALTHY CHILDREN OF DIFFERENT AGE GROUPS AND CONDITIONALLY HEALTHY ADULT INDIVIDUALS

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Abstract

Neutrophilic granulocytes (NG) with different receptor equipment are heterogeneous subpopulations that have differences in their biological properties and role in the pathogenesis of immune diseases. In result of research we obtained new data on the phenotypic features of NG subpopulations carrying the CD64, CD32, CD16 and CD11b molecules in newborns and children compared to the group of adult healthy individuals. It has been established that in the peripheral blood of healthy full-term newborns, conditionally healthy children of different age and conditionally healthy adults there are two main subpopulations of circulate NG – major with СD64–CD16+CD32+CD11b+ phenotype and minor СD64+CD16+CD32+CD11b+ phenotype, different receptor expression density noted in both subpopulations. It was shown that each of the studied groups has its own phenotypic profile, possibly optimal for performing NG eff ector functions. In newborns are identified phenotypes СD64–CD16dimCD32dimCD11bbrightNG and СD64dimCD16midCD32bright CD11bbrightNG, in children 2–4 years old СD64–CD16brightCD32dimCD11bbrightNG and СD64midCD16bright CD32dimCD11bbright NG, in children 5–9 years old СD64–CD16midCD32dimCD11bdimNG and СD64dim CD16brightCD32midCD11bdimNG, in conditionally healthy adults СD64–CD16midCD32dimCD11bdimNG and СD64brightCD16dimCD32midCD11bdimNG. Study shown that the NG of newborns and children of different age groups have a similar expression density of the CD64, CD16, CD32, CD11b receptors in major and minor subpopulations, NG subpopulations of healthy adults are equipped differently. The study of receptors in physiological according to age-related features is necessary for the correct interpretation of the transformation of the phenotype, changes in surface receptors expression in various pathological conditions. 

About the authors

G. A. Chudilova

Federal State Budget Educational Institution of Higher Education «Kuban State Medical University» of Ministry of Health Development of Russia

Author for correspondence.
Email: chudilova2015@yandex.ru

PhD (Biological Sciences), Associate Professor, Head of the Department of Clinical and Experimental Immunology and Molecular Biology of the Central Research Laboratory,

Krasnodar

Russian Federation

I. V. Nesterova

Federal State Budget Educational Institution of Higher Education «Kuban State Medical University» of Ministry of Health Development of Russia;
Federal State Budget Educational Institution of Higher Professional Education “Peoples’ Friendship University of Russia” of Ministry of Education and Science of Russia

Email: fake@neicon.ru

Chief Researcher of Department of Clinical and Experimental Immunology and Molecular Biology of Central Research Laboratory;

Doctor of Medical Sciences (DM), Professor, Professor of the Department of Allergology and Immunology,

Krasnodar

Russian Federation

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