REGULATORY CD4+ T CELLS ISOLATED FROM NAIVE, MEMORY AND EFFECTOR LYMPHOCYTE SUBSETS KEEP THE PROPERTIES OF APPROPRIATE SUBPOPULATIONS

In the blood of healthy volunteers, CD4⁺ T-lymphocyte subsets (naïve cells, central and eff ector memory cells, terminally diff erentiated effectors) were determined by flow cytometry. Using CD25, CD127, and FoxP3 molecules, regulatory T-lymphocytes (Tregs) were identified within each subset. Mitochondrial mass and charge, as well as the expression of CD71, CD39, GARP, LAP, and transcriptional co-activator PGC-1α regulating energy metabolism were assessed within each subset’s total cell pool and Tregs isolated from the appropriate subset. Correlation analysis showed that the Treg activation phenotype reflects the one of the parent subset. Furthermore, strong links between the energy indices of Tregs and the appropriate T-cell subset were established.