Parameters of interferon system, matrix metalloproteinases and related tissue inhibitors in women with papillomavirus infection during preconceptional preparation

Pregravid or preconceptional preparation comprises one of important aspects in planing pregnancy and labor that includes a set of diagnostics as well as vurative and preventive measures aimed at ensuring conception and birth of healthy children. Currently, preconceptional preparation is viewed crucial for successful conception, both provided by a woman as well as a man. Virus-associated infection is one of the main causes resulting in impaired reproductive function. Host immune response in the urinary tract is often manifested as bacterial vaginosis (BV) underlying sufficient enviroinment for developing ascending inflammatory process that accounts for its intensity and duration and lead to reproductive disorders.
Our study provides the data on interferon system, matrix metalloproteinases and related tissue inhibitors in women with STDs and/or papillomavirus infection.
The aim of the study was to assess level of interferons, components of matrix metalloproteinases and related tissue inhibitors in women with papillomavirus infection during preconceptional preparation.
There were examined 73 patients for infection with sexually transmitted diseases (STDs) in accordance with standard protocol. All patients were divided into three groups: Group I (n = 32) – STDs with papillomavirus infection (PVI); Group II (n = 27) – STDs without papillomavirus infection and Group III – Control, consisting of 14 apparently healthy female volunteers. The average age of the patients and control subjects was 26.3±3.2 and 29.5±2.2 years, respectively. Blood serum IFN, MMP and TIMP levels were measured by using specific reagents (R&D Diagnostics Inc., USA) with sandwich ELISA, according to the manufacturer’s instructions, on Multiskan immunoassay analyzer (Finland), presented as pg/ml and ng/ml. SPSS v. 20 software was used for the statistical analysis with non-parametric method.
Disturbances in type 1, 2 and 3 interferons were found in women with papillomavirus infection. An increased amount of MMP-2,8,9 and related tissue inhibitors TIMP-1 and TIMP-3 in women with PVI was revealed. Long-term HPV persistence leads to developing chronic systemic inflammatory response manifested as immune-inflammatory syndrome accounting for tissue morphological changes making impossible onset of pregnancy.