INVESTIGATION OF BIOSPECIFIC INTERACTION BETWEEN DNA-APTAMER AND CARBON NANOPARTICLES

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Abstract

Aptamers are DNA- and RNA-oligonucleotides that can selectively bind target molecules. Objective of our work was to investigate into interaction of biotinylated DNA-aptamer specific to human IgE with carbon nanoparticles covalently conjugated with streptavidin. Maximal level of specific interaction (about 40 pM per 1 mg of nanoparticles) was observed when concentration of aptamer was 100 nM.

About the authors

M. BA Rayev

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

P. V.1· Khramtsov

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Email: noemail@neicon.ru
Russian Federation

M. S. Bochkova

Institute of Ecology and Genetics of Microorganisms UB RAS

Email: noemail@neicon.ru
Russian Federation

V. P. Timganova

Institute of Ecology and Genetics of Microorganisms UB RAS

Email: noemail@neicon.ru
Russian Federation

S. A.1· Zamorina

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

Email: noemail@neicon.ru
Russian Federation

M. D. Kropaneva

Perm State National Research University

Email: noemail@neicon.ru
Russian Federation

References

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  2. Posthuma-Trumpie G., Wichers J., Koets M., Ber-endsen L, van Amerongen A. Amorphous carbon nanoparticles: a versatile label for rapid diagnostic (immuno)assays. Analytical and Bioanalytical Chemistry. 2012, 402 (suppl.), 593-600.
  3. Wiegand T. W., Williams P. B., Dreskin S. C., Jou-vin M. H., Kinet J. P., Tasset D. J. Immunol. High-affinity oligonucleotide ligands to human IgE inhibit binding to Fc epsilon receptor I. 1996, 157 (suppl.), 221-30.
  4. Раев М. Б., Храмцов П. В., Бочкова М. С. Исследование размеров углеродных наночастиц, ковалентно функционализированных белковыми макромолекулами. Российские Нанотехнологии. 2015, 1-2, 112-118.
  5. В. П. Тимганова, М. С. Бочкова, М. Б. Раев. Стабильность структурно-функциональных свойств углеродных диагностикумов. Доклады академии наук. 2013, 450, 4, 492-495.

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Copyright (c) 2017 Rayev M.B., Khramtsov P.V., Bochkova M.S., Timganova V.P., Zamorina S.A., Kropaneva M.D.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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