FEATURES OF OXIDOREDUCTASE, AMYLASE AND ATPASE ACTIVITIES OF IgG ANTIBODIES FROM BLOOD OF PATIENTS WITH SCHIZOPHRENIA

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Abstract

Electrophoretically and immunologically homogeneous IgG preparations were isolated from blood plasma of 18 patients with schizophrenia (SHZ) and 14 healthy donors by affinity chromatography on Protein G-Sepharose with subsequent high-performance gel-filtration. H2O2-dependent peroxidase and H2O2-independent oxidoreductase activities of polyclonal IgG of SHZ patients and healthy donors in the oxidation of 3,3’-diaminobenzidine were compared for the first time. All IgG antibodies of SHZ patients and healthy donors possess these activities, but the apparent kcat values varied in a very wide range (16,2-355,8 min–1). The average oxidation rates of the substrate in the presence of H2O2 from the plasma of SHZ patients and healthy donors were 1,3-1,5 times higher than in the absence of H2O2. The difference between the average peroxidase (1,8 times) and oxidoreductases (1,5 times) activities of IgG from blood plasma of SHZ patients and healthy donors was statistically significant (p = 0,008). The correlation coefficient of oxidoreductase and peroxidase activities of antibodies of patients with SHZ was significantly higher (0,664) than for healthy donors (0,27). A possible biological role and the nature of abzymes origin with oxidoreductase functions are discussed. The blood of healthy donors and patients with various autoimmune diseases usually contains abzymes with amylase and ATPase activities. In the case of 18 patients with SHZ amylase activity was detected only for one IgG preparation, but all 18 IgGs were inactive in the hydrolysis of ATP.

About the authors

E. A. Ermakov

Institute of Chemical Biology and Fundamental Medicine, Siberian branch of the Russian Academy of Sciences; Scientific Research Institute of Mental Health of the Tomsk National Research Medical Center of the Russian Academy of Sciences

Email: fake@neicon.ru

post-graduate student

Novosibirsk Russian Federation

A. S. Tolmacheva

Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences

Email: fake@neicon.ru

post-graduate student

Novosibirsk Russian Federation

L. P. Smirnova

Scientific Research Institute of Mental Health of the Tomsk National Research Medical Center of the Russian Academy of Sciences

Email: fake@neicon.ru

Ph.D., Senior Researcher

Tomsk

Russian Federation

S. A. Ivanova

Scientific Research Institute of Mental Health of the Tomsk National Research Medical Center of the Russian Academy of Sciences

Email: fake@neicon.ru

Doctor of Sciences (Medicine), Professor, Head. Laboratory

Tomsk

Russian Federation

V. N. Buneva

Institute of Chemical Biology and Fundamental Medicine, Siberian branch of the Russian Academy of Sciences

Email: fake@neicon.ru

Doctor of Sciences (Biology), Professor, Chief Researcher 

Novosibirsk

Russian Federation

G. A. Nevinsky

Institute of Chemical Biology and Fundamental Medicine, Siberian branch of the Russian Academy of Sciences

Author for correspondence.
Email: nevinsky@niboch.nsc.ru

Doctor of Sciences (Chemistry), professor, Head of Laboratory

630090 Novosibirsk, Lavrentiev Ave., 8

Russian Federation

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