CHARACTERISTICS OF IMMUNE RESPONSE INDUCED BY GMDP PEPTIDE MIMETIC RN: A COMPARATIVE ANALYSIS WITH MURAMYLPEPTIDES

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Abstract

To study the mechanism of action of the GMDP peptide mimetic RN, its eff ect on the expression level of mRNA of the pro-infl ammatory cytokines IL-1β and TNF-α and some molecules involved in the activation of the NF-kB signaling pathway in human leukemic monocyte cells THP-1 was evaluated. For comparison, GMDP itself and its two analogues with well-characterized biological activity, GMDPacid and GMDP-Lys, were used. All compounds demonstrated signifi cant diff erences during the development of the immune response: GMDP-Lys turned out to be the strongest stimulator of the immune response; GMDP-acid to a greater extent enhanced the expression of the p100/p52 subunit (NF-kB2) and to a lesser extent the expression of IL-1β in comparison with GMDP; the peculiarities of peptide RN response were low level of IL-1β production and stimulation of the adapter protein RIP2 of innate immunity NOD2 receptor. All compounds studied are valuable tools for fi nding new ways for immune response correction.

About the authors

L. G. Alekseeva

M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, RAS

Author for correspondence.
Email: luda.alekseeva@mail.ru

Ph.D, senior researcher, Laboratory of Peptide Chemistry,

117997, Moscow, Miklukho-Maklaya ul., 16/10

Russian Federation

E. A. Meshcheryakova

M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, RAS

Email: fake@neicon.ru

Ph.D., researcher, Laboratory of Peptide Chemistry,

Moscow

Russian Federation

A. G. Laman

Branch of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, RAS

Email: fake@neicon.ru

senior researcher, Laboratory of Immunochemistry,

Pushchino, Moscow region

Russian Federation

T. M. Andronova

Joint-Stock Company “PEPTEK”

Email: fake@neicon.ru

Ph.D., president,

Moscow

Russian Federation

V. T. Ivanov

M.M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, RAS

Email: fake@neicon.ru

academician, Leader of Laboratory of Peptide Chemistry,

Moscow

Russian Federation

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Copyright (c) 2019 Alekseeva L.G., Meshcheryakova E.A., Laman A.G., Andronova T.M., Ivanov V.T.

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