Email this article THE RELATIONSHIP OF BIOMARKERS AND PLATELET AGGREGATION ACTIVITY ON THE BACKGROUND OF HYDROGEN SULFIDE IN PATIENTS WITH CORONARY HEART DISEASE
- Authors: Trubacheva, O.A.1, Gusakova A.M.2, Schneider O.L.2, Birulina J.G.1, Chumakova S.P.1, Petrova I.V.1
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Affiliations:
- Siberian State Medical University
- Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
- Section: SHORT COMMUNICATIONS
- URL: https://rusimmun.ru/jour/article/view/16577
- DOI: https://doi.org/10.46235/1028-7221-16577-TRO
- ID: 16577
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Abstract
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Abstract
The relationship of biomarkers and platelet aggregation activity on the background of hydrogen sulfide in patients with coronary heart disease
Objective: to determine concentrations and identify the relationship of biomarkers (LIGHT, PlGF, IFN α2, TNF-αβ, IL-3, IL-5, IL-6, IL-8, IL-15, IL-17F, MIP-1α, CXCL16) with indicators of collagen-induced platelet aggregation against the background of action hydrogen sulfide in patients with coronary heart disease. The study included 22 patients with coronary heart disease. The level of biomarkers was determined by multiplex analysis. Platelet aggregation was studied by the turbodimetric method. The samples were examined against the background of a 30-minute preincubation with hydrogen sulfide and an inducer of collagen aggregation at a concentration of 2 mmol/L. Sodium hydrosulfide was used as a hydrogen sulfide donor at a concentration of 10-6 M. Patients were divided into two groups: group 1 (n=10) - the degree of aggregation or the size of aggregates decreased against the background of preincubation with hydrogen sulfide and group 2 (n=12) - preincubation with hydrogen sulfide led to an increase in the degree or size of aggregates. Correlation analysis revealed that correlations were found in group 1 between the concentrations of MIP-1a, IL-5 and IL-8, respectively, both with the size of aggregates and with the degree of platelet aggregation. In group 2, correlations were found with the size of aggregates and PIGF (Rs=0.59, p=0.04) and with CXCL16 both with the size of aggregates (Rs=0.58, p=0.04) and with the degree of aggregation (Rs=0.65, p=0.04). It was found that group 2 had higher concentrations of inflammatory biomarkers (IFN α2, IL-3, IL-8, IL-15, IL-17F, MIP-1α). It is probably the more pronounced proinflammatory status in this group of patients that leads to platelet inertia in relation to the inhibitory effect of hydrogen sulfide. In the 1st group of patients, multiple correlations of aggregation parameters with inflammatory markers (IL-5, IL-6, IL-8, MIP-1a) were revealed, which may lead to the use of various corrective therapies for patients of these groups.
About the authors
Oksana Alexandrovna Trubacheva,
Siberian State Medical University
Email: otrubacheva@inbox.ru
ORCID iD: 0000-0002-1253-3352
Candidate of Medical Sciences, Associate Professor of the Department of Physical Culture and Health
Russian Federation, 634050, Russian Federation, Tomsk, Moskovsky tract, 2Anna Mikhailovna Gusakova
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
Email: mag_a@mail.ru
ORCID iD: 0000-0002-3147-3025
Candidate of Pharmaceutical Sciences, Senior Research Fellow, Department of Clinical Laboratory Diagnostics
Russian Federation, 634012 Russian Federation str., Tomsk, Kievskaya 111aOlga Leonidovna Schneider
Cardiology Research Institute, Tomsk National Research Medical Centre, Russian Academy of Sciences, Tomsk, Russia
Email: shnaide65@mail.ru
ORCID iD: 0000-0003-2461-1423
Junior Researcher at the Department of Atherosclerosis and Chronic Ischemic Heart Disease
Russian Federation, 111a Kievskaya str., Tomsk, 634012, Russian FederationJulia Georgievna Birulina
Siberian State Medical University
Email: birulina20@yandex.ru
ORCID iD: 0000-0003-1237-9786
PhD. Biol. sciences, Associate Professor, Department of Biophysics and Functional Diagnostics
Russian Federation, 2, Moskovsky tract, Tomsk, 634050, Russian FederationSvetlana Petrovna Chumakova
Siberian State Medical University
Email: chumakova_s@mail.ru
ORCID iD: 0000-0003-3468-6154
Doctor of Medical Sciences, Professor, Department of Pathophysiology
Russian Federation, 634050, Российская Федерация, г. Томск, Московский тракт, 2Irina Viktorovna Petrova
Siberian State Medical University
Author for correspondence.
Email: ivpetrova57@yandex.ru
ORCID iD: 0000-0001-9034-4226
Doctor of Biological Sciences, Professor, Department of Biophysics and Functional Diagnostics
Russian Federation, 2, Moskovsky tract, Tomsk, 634050, Russian FederationReferences
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