RELATIONSHIP OF THE PHENOTYPE OF PERIPHERAL BLOOD LYMPHOCYTES AND SIGNS OF OSTEOPENIA IN PATIENTS WITH CHRONIC LYMPHOLIC LEUKEMIA



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Abstract

AbstractChronic lymphocytic leukemia (CLL) is the most common leukemia among adults in Western countries, characterized by the development of a number of complications, including osteoporosis, which is a prerequisite for studying its predictors. The purpose of the work is to investigate the relationship between immunophenotyping indicators of blood lymphocytes and osteodensitometry indicators in CLL. The study was conducted on 48 male patients with CLL aged 50-70 years with an average disease duration of 12 months and 14 apparently healthy men of the corresponding age (group 1). In the blood, CD5+, CD19+, CD20+, CD22+, CD23+, CD43+, CD200+ lymphocytes were differentiated in the blood using flow cytometry. The international FRAX questionnaire was used to assess the probability of fracture. Osteodensitometry assessed bone mineral density (BMD), T-score (standard deviation, SD), Z-score (SD) in the lumbar spine (LS), proximal femoral neck (FNA), proximal femoral bone (PFB). Patients with CLL were included in group 2 (n=34) with BMD within normal limits (T- and Z-score > – 1.0 SD) or group 3 (n=14) with signs of osteopenia (OP) (T- and Z-score from – 1.0 SD up to – 2.5 SD). In patients with CLL in group 3, compared with patients with CLL in group 2, there is a significant increase in the number of lymphocytes in the blood expressing markers CD5+, CD19+, CD20+, CD22+, CD23+, CD43+, CD200+. In patients with CLL in group 3, signs of AP in the SPBC increase with the increase in lymphocytes with the CD5+, CD19+, CD20+, CD22+, CD23+, CD43+, CD200+ phenotype in the blood. Signs of AP in the PBC increase with the increase in lymphocytes with the CD5+, CD19+, CD20+, CD23+, CD43+ phenotype in the blood. The strongest connections were found between the number of lymphocytes in the blood with the markers CD5+, CD19+, CD23+, CD43+ and the T-score, Z-score, and BMD in the FNA. The data obtained are a prerequisite for further study of the interaction between the clone of tumor lymphocytes in CLL and cells involved in bone tissue remodeling to identify the mechanism of development of OP, osteoporosis and the risk of bone fractures, and changes in the quantitative composition of the lymphocyte phenotype in the blood can be considered as potential laboratory predictors of a decrease in BMD.

About the authors

Mikhail V. Osikov

South State Medical University, Chelyabinsk, Russia;
Chelyabinsk Regional Clinical Hospital, Chelyabinsk, Russia

Email: prof.osikov@yandex.ru
ORCID iD: 0000-0001-6487-9083
SPIN-code: 7919-2947
Scopus Author ID: 16040195100
ResearcherId: DWQ-4192-2022

Dr. Sc. (Med.), Head of Pathophysiology Department at the South State Medical University; Head of scientific department at the Chelyabinsk Regional Clinical Hospital. 

Russian Federation, South State Medical University, Chelyabinsk, Russia; Chelyabinsk Regional Clinical Hospital, Chelyabinsk, Russia

Egor A. Korobkin

South State Medical University, Chelyabinsk, Russia;
Chelyabinsk Regional Clinical Hospital, Chelyabinsk, Russia

Email: doktore77@yandex.ru
ORCID iD: 0000-0001-7241-1325
SPIN-code: 4652-3530
Scopus Author ID: 58698243600
ResearcherId: HZK-1427-2023

Assistant Professor of Pathophysiology Department at the South State Medical University; Hematologist at the Chelyabinsk Regional Clinical Hospital.

 

Russian Federation, South State Medical University, Chelyabinsk, Russia; Chelyabinsk Regional Clinical Hospital, Chelyabinsk, Russia

Aleksey A. Fedosov

Peoples' Friendship University of Russia (RUDN University), Moscow, Russia

Email: fedosov.76@mail.ru
ORCID iD: 0000-0003-2224-5175
SPIN-code: 1828-0622

Cand. Sc. (Med.), Associate Professor at the Department of Histology, Cytology and Embryology

Russian Federation, 117198, Russia, Moscow, st. Miklouho-Maklaya, 6

Georgy P. Dimov

South State Medical University, Chelyabinsk, Russia

Author for correspondence.
Email: first-aid03@yandex.ru
SPIN-code: 2073-9645

Cand. Sc. (Med.), researcher, Research Institute of Immunology at the South State Medical University, Head of the department of collection, procurement and storage of hematopoietic cells and bone marrow of the clinic of the South State Medical University.

Russian Federation, South State Medical University, Chelyabinsk, Russia

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Copyright (c) Osikov M.V., Korobkin E.A., Fedosov A.A., Dimov G.P.

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