THE LEVEL OF SOME MATRIX METALLOPROTEINASES IN BLOOD SERUM IN RELATION TO THEIR GENETIC POLYMORPHISM DEPENDING ON AGE

AbstractThe article presents data on the state of the proteolysis system and genes for the regulation of matrix metalloproteinases in 180 conditionally healthy women and men of young, middle, elderly and senile ages. A single sampling of the material (buccal epithelium) was performed in patients. PCR examination of the buccal epithelium for the analysis of polymorphisms of the genes MMP1 rs1799750 and MMP3 rs 3025058 was carried out on the instrument base of the DNA amplifier Bio-Rad CFX96. Serum levels of MMP-1,-3 were studied by the sandwich variant of solid-phase enzyme immunoassay. Statistical processing of the obtained results was carried out using the IBM SPSS Statistics 26 program using nonparametric statistics methods. The results of the study. The values of MMR-1 and MMR-3 in the group of conditionally healthy women at a young age were lower than the values of middle age, whereas in old age their serum levels varied in different directions: MMR-1 decreased, and MMR-3 increased. When analyzing polymorphisms of the MMR-1 rs1799750 gene, the homozygous genotype -/- with low expression of the MMR-1 gene prevailed in young and middle-aged women, and in women over 75 years of age, the carriage of the G/G homozygote with active gene expression was recorded. When evaluating the expression of the MMR-3 rs 3025058 gene, it was found that young women significantly more often had 5A/5A polymorphism with high gene activity, but with a lower level of MMR-3. In middle and old age, 65% have a heterozygous 5A/6A allele with reduced gene expression activity. In conditionally healthy men, the level of MMR-1 in blood serum increased in the middle-aged and senile groups, the values of MMR-3 in the group under 45 years of age were low compared with the elderly group (p=0.041). MMR-1 rs1799750 in men under 75 years of age was identified in the -/G variant in the majority. Homozygous polymorphism of the MMR-3 rs 3025058 5A/5A gene with high activity of MMR-3 prevailed in groups of men under 60 years of age with serum levels of MMR-3 100.2 ng/ml and 92.5 ng/ml, respectively.