TISSUE POOL OF HYPERSEGMENTED NEUTROPHILS IN PATIENTS WITH PRECANCEROUS AND TUMOR DISEASES OF THE LARYNX AND LARYNGOPHARYNX



Cite item

Full Text

Abstract

Abstract

The presence of neutrophils in a tumor often correlates with an unfavorable prognosis, however, there is still no clear answer about the role of tumor-associated neutrophils and the relationship of their morphofunctional features with the prognosis of the course of the disease. The aim of the study was to evaluate the structural features of tissue neutrophil nuclei in the pathological zone in patients with precancerous changes and malignant neoplasms in the larynx. 8 people with precancerous changes in the larynx, 18 patients with localized cancer of the larynx (stages II and III of the tumor process and the absence of metastases - T2-3N0M0) and 12 patients with advanced cancer (stage III of tumor diseases and regional metastases - T3N1-2M0) were examined. Blood slides and smear prints of tissue biopsies from three localizations were examined: 1 - zones of the pathological focus; 2 - boundaries between the pathological focus and conditionally healthy tissue; 3 – conditionally healthy tissue. In the blood of patients with precancerous changes in the larynx and cancer patients, the main part of neutrophils was represented by cells with a 4-segment nucleus, and the number of hypersegmented forms (5 or more segments) was higher than in the blood of  healthy volunteers (p<0,002). The same type of changes in smear prints of biopsies from different areas of the pathological focus in patients with precancerous and malignant neoplasms were revealed: as they approached the pathological focus, an increase the cells whith 4-5 or more segments was observed. The content of hypersegmented forms was maximum in the pathological focus. Only in patients with a widespread tumor process, neutrophils with a 4-segment nucleus dominated the immediate environment of the tumor. As the tumor spreads and metastases form, the proportion of hypersegmented cells increases in the population of intra-tumor neutrophils. It is very likely that the morphological features of tissue neutrophils in different zones of the pathological focus reflect their functional heterogeneity, and hypersegmetation of nuclei can be considered as a potential predictor of the development and progression of the tumor process.

About the authors

Elena N. Kologrivova

Siberian State Medical University

Email: enkologrivova@mail.ru

Dr. Sci. (Med.),  Prof., Prof. of  the Department of Immunology and Allergology

Russian Federation

Raisa I. Pleshko

Siberian State Medical University

Email: raisap57@mail.ru

M.D., Ph.D., Dr. Sci. (Med.),  Prof., Prof. of the Department of Morphology and General Pathology, Siberian State Medical University, Tomsk, Russian Federation

Russian Federation

Olga V. Cheremisina

Cancer Research Institute, Тomsk National Research Medical Center, Russian Academy of Sciences (Tomsk, Russia)

Email: cheremisinaov@oncology.tomsk.ru

M.D., Ph.D., Dr. Sci. (Med.), Head of Endoscopy Department, Cancer Research Institute, Тomsk National Research Medical Center, Russian Academy of Sciences (Tomsk, Russia)

Russian Federation

Margarita A. Boldyshevskaya

Cancer Research Institute, Тomsk National Research Medical Center, Russian Academy of Sciences (Tomsk, Russia)

Email: afanasyeva97@yandex.ru

Doctor of Clinical Laboratory Diagnostics of the Pathological and Anatomical Department, Cancer Research Institute, Тomsk National Research Medical Center, Russian Academy of Sciences (Tomsk, Russia)

Russian Federation

Elena A. Gereng

Siberian State Medical University

Email: e-gereng@mail.ru

M.D., Ph.D., Dr. Sci. (Med.),  Prof. of the Department of Morphology and General Pathology, Siberian State Medical University, Tomsk, Russian Federation

Russian Federation

Temur Nasibov

Siberian State Medical University

Author for correspondence.
Email: temur.nsbv@gmail.com

fifth-year student, Siberian State Medical University, Tomsk, Russian Federation

Russian Federation

References

  1. Долгушин И.И., Мезенцева Е.А. Нейтрофильные гранулоциты: участие в гомеостатических и репаративных процессах. Часть II // Инфекция и иммунитет.- 2021. -Т. 11, № 1.- С. 25–41. Dolgushin I.I., Mezentseva E.A. Neutrophil granulocytes: participation
  2. in homeostatic and reparative processes. Part II // Russian Journal of Infection
  3. and Immunity = Infektsiya i immunitet, 2021, Vol. 11, no. 1, pp. 25–41.
  4. https://iimmun.ru/iimm/article/view/1258 [doi: 10.15789/2220-7619-NGP-1258]
  5. Кологривова Е.Н., Плешко Р.И., Черемисина О.В., Болдышевская М.А. «Гиперсегментация ядер нейтрофилов крови у больных с локализованными распространенным раком гортани и гортаноглотки»// Медицинская иммунология. - 2023.- Т. 25, № 5. -С. 1111-1116. Kologrivova E.N. Pleshko R.I., Cheremisina O.V.,
  6. Boldyshevskaya M.A. “Hypersegmentation of neutrophil
  7. nuclei in peripheral blood of patients with localized and
  8. advanced cancer of the larynx and laryngopharynx”, Medical
  9. Immunology (Russia)/Meditsinskaya Immunologiya, 2023,
  10. Vol. 25, no. 5, pp. 1111-1116.
  11. https://www.mimmun.ru/mimmun/article/view/2715/1769 [doi: 10.15789/1563-0625-HON-2715]
  12. Fridlender Z.G., Sun J., Kim S., Kapoor V., Cheng G., Ling L., Worthen G.S., Albelda S.M. Polarization of tumor-associated neutrophil phenotype by TGF-beta: "N1" versus "N2" TAN. Cancer Cell, 2009, Sep 8, Vol.16, no.3, pp.183-94. - https://www.cell.com/cancer-cell/fulltext/S1535-6108(09)00215-3 [doi: 10.1016/j.ccr.2009.06.017. PMID: 19732719; PMCID: PMC2754404]
  13. Fridlender Z.G., Albelda S.M. Tumor-associated neutrophils: friend or foe? Carcinogenesis, 2012,Vol. 33, no.5, pp.949-55. - https://academic.oup.com/carcin/article/33/5/949/2463833?login=false [doi: 10.1093/carcin/bgs123. Epub 2012 Mar 16. PMID: 22425643]
  14. Fridlender Z.G., Granot Z. Neutrophils in the tumor microenvironment - when a company becomes a crowd. Cell Mol Immunol, 2024, Mar 8. - https://www.nature.com/articles/s41423-024-01147-9 [doi: 10.1038/s41423-024-01147-9. Epub ahead of print. PMID: 38459242]
  15. Jaillon S., Ponzetta A., Di Mitri D., Santoni A,, Bonecchi R., Mantovani A. Neutrophil diversity and plasticity in tumour progression and therapy. Nat Rev Cancer, 2020, Vol.20, no.9, pp.485-503. - https://www.nature.com/articles/s41568-020-0281-y [doi: 10.1038/s41568-020-0281-y. Epub 2020 Jul 21. PMID: 32694624]
  16. Manley H.R., Keightley M.C., Lieschke G.J. The neutrophil nucleus: an important influence on neutrophil migration and function. Front. Immunol., 2018, Vol.9, 2867. - https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.02867/full [doi: 10.3389/fimmu.2018.02867]
  17. Masucci M.T., Minopoli M., Carriero M.V. Tumor associated neutrophils. Their role in tumorigenesis, metastasis, prognosis and therapy. Front. Oncol., 2019, Vol.9, 1146. - https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2019.01146/full [doi: 10.3389/fonc.2019.01146]
  18. Mishalian I, Granot Z, Fridlender ZG. The diversity of circulating neutrophils in cancer. Immunobiology. 2017, Vol.222, no.1, pp.82-88. - https://www.sciencedirect.com/science/article/abs/pii/S0171298516300171?via%3Dihub [doi: 10.1016/j.imbio.2016.02.001. Epub 2016 Feb 3. PMID: 26874580]
  19. Murdoch C., Muthana M., Coffelt S.B., Lewis C.E. The role of myeloid cells in the promotion of tumour angiogenesis. Nat Rev Cancer, 2008, Vol. 8, no.8, pp.618-631. - https://www.nature.com/articles/nrc2444 [doi: 10.1038/nrc2444. Epub 2008 Jul 17. PMID: 18633355]
  20. Ng M.S.F., Kwok I., Tan L., Shi C., Cerezo-Wallis D., Tan Y., et al. Deterministic reprogramming of neutrophils within tumors. Science, 2024, Vol.12, no.383, 6679. - https://www.science.org/doi/10.1126/science.adf6493?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed [doi: 10.1126/science.adf6493. Epub 2024 Jan 12. PMID: 38207030]
  21. Ohms M., Moller S., Laskay T. An attempt to polarize human neutrophils toward N1 and N2 phenotypes in vitro. Front. Immunol., 2020, Vol. 11, 532. - https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2020.00532/full [doi: 10.3389/fimmu.2020.00532]
  22. Powell D.R., Huttenlocher A. Neutrophils in the Tumor Microenvironment. Trends Immunol., 2016, Vol.37, no.1, pp.41-52. - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4707100/ [doi: 10.1016/j.it.2015.11.008. Epub 2015 Dec 14. PMID: 26700397; PMCID: PMC4707100]
  23. Salvermoser M., Begandt D., Alon Rand Walzog B. Nuclear Deformation During Neutrophil Migration at Sites of Inflammation.Front. Immunol., 2018, Vol.9, 2680. - https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2018.02680/full [doi: 10.3389/fimmu.2018.02680]
  24. Uribe-Querol E., Rosales C. Neutrophils in Cancer: Two Sides of the Same Coin. J Immunol Res., 2015, Vol. 2015, 983698. - https://www.hindawi.com/journals/jir/2015/983698/ [doi: 10.1155/2015/983698. Epub 2015 Dec 24. PMID: 26819959; PMCID: PMC4706937]

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) Kologrivova E., Pleshko R., Cheremisina O., Boldyshevskaya M., Gereng E., Nasibov T.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № 77 - 11525 от 04.01.2002.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies