ANALYSIS OF THE RAT JAW BONE TISSUE ELEMENTAL COMPOSITION IN A MODEL OF PERIODONTITIS



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Abstract

Abstract

The elemental composition of the jaw bone tissue depends on many external and internal factors affecting the body. The structure of bone tissue directly reflects its ability to restrain inflammation and regenerate after exposure to damaging factors.

The purpose of the study is to study the elemental composition of the rat jaw bone tissue during chronic periodontal inflammatory process. A month later, the individual was removed from the experiment by an overdose of ether to isolate the bone segment under study. Next, the bone tissue sample was studied using atomic emission spectroscopy.

In the studied segment of bone tissue, 67 chemical elements were identified in a quantitative range of up to 10-4 mass percent. The highest mass fraction belongs to the basic elements of bone tissue: calcium, phosphorus, magnesium, sodium, sulfur. While during the experiment the animals received standard complete feed, which contained proteins, vitamin D close to normal , Ca, P and Se. In the bone tissue sample under study, the quantitative Ca/P ratio was 1.685, which slightly exceeds the optimal one. An increase in this indicator is associated with a decrease in phosphorus content in bone tissue, which can be explained by insufficient daily intake of this element from food, as well as a number of local factors. When the jaw is traumatized during the modeling of periodontitis, an inflammatory process occurs in this area, the experimental animal experiences stress, which leads to demineralization of the tissues of the alveolar process of the jaw, as evidenced by a decrease in the content of phosphorus and selenium in the bone tissue. In addition, elemental analysis showed the presence of foreign elements in the bone tissue, the amount of which corresponds to the range from 0.001% to 0.06%. Basically, these are elements that form simple substances - metals: bismuth, gallium, lead, iridium, titanium, zinc, mercury, molybdenum.

The results of the study can be taken into account when planning and conducting polyetiotropic therapy for inflammatory-dystrophic periodontal diseases in the early stages of their development.

About the authors

Narine Grishaevna Sarkisyan

Ural state medical university, Yekaterinburg, Russian Federation;
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russian Federation

Email: narine_25@mail.ru

Associate Professor of the Department of Therapeutic Dentistry and Propedeutics of Dental Diseases, Doctor of Medical Sciences, professor of postgraduate department

Russian Federation

Natalia Nikolaevna Kataeva

Ural state medical university, Yekaterinburg, Russian Federation

Email: kataeva.nn@mail.ru
ORCID iD: 0000-0002-2847-8810

PhD, associate professor, assistant professor of General Chemistry department

Russian Federation

Diana Aleksandrovna Khokhryakova

Ural state medical university, Yekaterinburg, Russian Federation

Email: diana_hohryakova@mail.ru

5th year student of the Dental Faculty

Russian Federation

Arsen Henrikovich Melikyan

Ural state medical university, Yekaterinburg, Russian Federation

Email: arsen.melikyan.01@mail.ru

5th year student of the Dental Faculty

Russian Federation

Izabella Mgerovna Оsipova

Ural state medical university, Yekaterinburg, Russian Federation

Author for correspondence.
Email: oim2001@bk.ru

5th year student of the Dental Faculty

Russian Federation

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Copyright (c) Sarkisyan N.G., Kataeva N.N., Khokhryakova D.A., Melikyan A.H., Оsipova I.M.

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