SYNTHETIC THYMIC HEXAPEPTIDE IN THE CORRECTION OF ALTERATIONS OF ANTIBACTERIAL IMMUNE DEFENSE AND NORMALIZATION OF THE PROFILE OF PROINFLAMMATORY CYTOKINES IN IMMUNOCOMPROMIZED CHILDREN WITH LOCAL UNLIMITED ACUTE PERITONITIS
- Authors: Kovaleva S.V.1, Chapurina V.N.1, Lomtatidze L.V.1, Lyagusha D.E.2, Teterin Y.V.1, Barova N.K.1,3, Melkonyan K.I.1
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Affiliations:
- FSBI HРE «KubSMU» MOH Russia, 350063, Krasnodar, Russian Federation
- Children’s Regional Clinical Hospital, Krasnodar, Russia
- Children’s Regional Clinical Hospital, Krasnodar, Russi
- Section: Joint Immunology Forum 2024
- URL: https://rusimmun.ru/jour/article/view/16743
- DOI: https://doi.org/10.46235/1028-7221-16743-STH
- ID: 16743
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Abstract
Abstract
The study of dysregulatory disorders of the immune system underlying the immunopathogenesis of severe purulent-inflammatory diseases (PIDs) is important for development of new therapeutic tactics of restoring antibacterial defense. Acute peritonitis (AP) is a severe PID of the abdominal cavity, the course of which are dependent on the treatment, cytokine balance and adequate functioning of the immunity. Objective: to evaluate the modulating effects on the immune system and the levels of pro-inflammatory cytokines of the synthetic thymic hexapeptide, active substance of Imunofan, included in the complex postoperative treatment (CPOT) of immunocompromised children with local AP. Clinical and immunological examination of 20 immunocompromised children aged 5-12 years with local AP was carried out before (study group 1, SG1) and after (study group 1a, SG1a) CPOT including synthetic thymic hexapeptide (Arginyl-alpha-Aspartyl-Lysyl-Valyl-Tyrosyl-Arginine), 20 conditionally healthy children (comparison group, SG). The content of T- and B-lymphocytes, natural killers (NK), levels of serum pro-inflammatory cytokines IL1β, IL6, TNFα, IL8, IL18, phagocytic and microbicidal activity of neutrophils (NG) were assessed. In SG1, before treatment, decrease in the number of T-lymphocytes, T-helpers, CTL-lymphocytes, NK and increase in the level of B-lymphocytes was revealed. Defects in the effector functions of NG were determined: impaired bacterial antigen killing and decreased NADPH oxidase activity. It was established that in case of AP in immunocompromised children, the cytokine profile is characterized by overproduction of studied pro-inflammatory and neutrophil-associated cytokines. After complex treatment including immunomodulatory therapy, there was a restoration of the content of T-lymphocytes, T-helper cells, TCTL-lymphocytes, an increase in the number of NK and decrease in the level of B-lymphocytes. In addition, regression of the levels of inflammatory, including neutrophil-associated, cytokines and emergence of effector functions of NG due to restoration of NADPH oxidases activity, was noted. Thus, the restoration of immunological parameters in AP leads to earlier regression of the purulent-inflammatory process in the abdominal cavity and to the absence of postoperative complications. The clinical and immunological effects of the immunomodulatory therapy program with inclusion of the drug based on HP determines the feasibility of its use in the postoperative period in immunocompromised children with local AP.
About the authors
Svetlana Valentinovna Kovaleva
FSBI HРE «KubSMU» MOH Russia, 350063, Krasnodar, Russian Federation
Email: 3483335@mail.ru
ORCID iD: 0000-0002-9604-5806
SPIN-code: 8289-5342
Scopus Author ID: 55535685400
ResearcherId: AAB-3408-2020
Doctor of Medical Sciences (MD), Associate Professor, senior researcher of the department of clinical and experimental immunology and molecular biology of the Central Scientific Research Laboratory, Associate Professor of the Department of Clinical Immunology, Allergology and Laboratory Diagnostics of FAT and PRS
Russian Federation, 4, st. Mitrofan Sedin, Krasnodar, 350063Valeriya Nikolayevna Chapurina
FSBI HРE «KubSMU» MOH Russia, 350063, Krasnodar, Russian Federation
Email: Pavlenkoevi2016@yandex.ru
ORCID iD: 0000-0002-1912-2038
PhD (Medical Sciences), Assistant of the Department of Clinical Immunology, Allergology and Laboratory Diagnostics of FAT and PRS, researcher of the department of clinical and experimental immunology and molecular biology of the Central Scientific Research Laboratory
Russian Federation, 4, st. Mitrofan Sedin, Krasnodar, 350063Lyudmila Viktorovna Lomtatidze
FSBI HРE «KubSMU» MOH Russia, 350063, Krasnodar, Russian Federation
Email: llomtatidze@mail.ru
ORCID iD: 0000-0002-7041-7106
PhD in Biology, senior researcher of the department of clinical and experimental immunology and molecular biology of the Central Scientific Research Laboratory, Associate Professor of the Department of Clinical Immunology, Allergology and Laboratory Diagnostics of FAT and PRS
Russian Federation, 350063, Krasnodar, Sedina street 4, Russian FederationDarina Eduardovna Lyagusha
Children’s Regional Clinical Hospital, Krasnodar, Russia
Email: darina_hapacheva@mail.ru
ORCID iD: 0009-0005-2919-4923
Pediatrician, Surgical Department No. 1
Russian Federation, 350007, Krasnodar, Pobedy Square, 1Yuri Valeryevich Teterin
FSBI HРE «KubSMU» MOH Russia, 350063, Krasnodar, Russian Federation
Email: yura.teterin.1989@mail.ru
ORCID iD: 0000-0002-3073-5070
Postgraduate Student, Department of Clinical Immunology, Allergology and Laboratory Diagnostics of FAT and PRS
Russian Federation, 4, st. Mitrofan Sedin, Krasnodar, 350063Natusya Kaplanovna Barova
FSBI HРE «KubSMU» MOH Russia, 350063, Krasnodar, Russian Federation;Children’s Regional Clinical Hospital, Krasnodar, Russi
Email: nbarova@yandex.ru
ORCID iD: 0000-0001-5857-2296
SPIN-code: 5365-0960
PhD (Medical Sciences), Associate Professor, Head of the Department of Surgical Diseases of Childhood; Head, Surgical Department No. 1
Russian Federation, 350063, Krasnodar, Sedina street 4, Russian Federation 350007, Krasnodar, Pobedy Square, 1Karina Igorevna Melkonyan
FSBI HРE «KubSMU» MOH Russia, 350063, Krasnodar, Russian Federation
Author for correspondence.
Email: kimelkonian@gmail.com
ORCID iD: 0000-0003-2451-6813
PhD (Medical Sciences), Head of Central Research Laboratory
Russian Federation, 350063, Krasnodar, Sedina street 4, Russian FederationReferences
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