DYNAMICS OF IMMUNOGRAM OF STUDENTS UNDER THE INFLUENCE OF DOSED PHYSICAL ACTIVITY

AbstractThe analysis of the dynamics of the state of blood leukocytes in muscular activity is relevant due to the development of methods of differentiation and assessment of the state of these cells. The purpose of the study is to study the dynamics of the phagocytic and NBT activity of neutrophils, as well as the content of CD-lymphocytes of peripheral blood in students when moving on tredmil. For students 18-20 years old involved in athletics (3 boys and 4 girls), blood was taken from the ulnar vein before and after a 15-minute jog, as well as after a 15-minute run of moderate intensity. The average level of physical performance of the examined PWC₁₇₀ was 16,25±2,21 kGm/min/kg. Assessment of phagocytosis and NBT activity of neutrophils and determination of blood CD-lymphocytes by immunophenotyping were performed using flow cytometry. It was found that in girls at rest, the relative content of lymphocytes and NK-cells was significantly lower, and the level of induced NBT activity of neutrophils and the relative content of CD₄^-CD₈^- and CD₄⁺CD₈⁺ lymphocytes was significantly higher than in boys. At the same time, the dynamics of the leukogram parameters examined during the dosed physical activity fully corresponded to the development of the lymphocytic phase of myogenic leukocytosis. With an increase in the power of repeated exercise against the background of a general increase in the number of phagocytic neutrophils, the balance of spontaneous NBT-positive and reserve potentially active neutrophils significantly increased relative to the content of NBT-negative cells in the induced test. At the same time, the level of neutrocytes in girls was significantly higher, causing a higher level of phagocyte count and absolute content of NBT-positive neutrophils in a spontaneous and induced test. At the same time, against the background of an increase in the absolute content of CD₄^-CD₈^- and TNK-lymphocytes, a statistically significant increase in the relative and absolute content of NK-cells was observed. In contrast to the boys after the 2nd physical exercise, the relative and absolute content of NK-cells, as well as the increase in the percentage of HBT-positive cells in the induced test, were significantly higher.