THE EFFECT OF THE PROBIOTIC MOUTHWASH "IMMUNITDOMINOS" ON THE CYTOKINE PROFILE AND ANTIMICROBIAL PROTEINS OF THE ORAL FLUID IN HEALTHY PEOPLE

Abstract

The search for probiotic strains with anti-inflammatory properties is an urgent problem in modern microbiology, immunology and biotechnology. Probiotic therapy is a new concept in dentistry. Probiotic drugs are available in a variety of commercial forms, including tablets, toothpaste, mouthwash. Probiotics affect immune function by regulating local and systemic mechanisms, interacting with epithelial cells of the oral cavity, thereby enhancing the barrier function of the mucous membrane, both in healthy people and with various dental pathologies. The aim of this study: to evaluate the effect of the probiotic rinse aid "ImmunitDominos" on the level of pro- and anti-inflammatory cytokines, immunoglobulins and lysozyme in oral fluid in healthy people.The material for the study was 64 oral fluid samples obtained from conditionally healthy people aged 30 to 76 years, who were divided into 2 groups during the work: 1- with initially low cytokine values in the oral fluid and 2- without somatic diseases, but with high cytokine values. The ingredient composition of the mouthwash "ImmunitDominos" was represented by a dry concentrate of five probiotic strains of bifidobacteria (the content of bifidobacteria in one sachet package is at least 10⁸ CFU/g). The mouthwash "ImmunitDominos" was used twice a day for 28 days. The levels of IgA, IgM, IgG (AO Vector-Best, Russia) and cytokines IFNγ, TNFα, IL-17, IL-6, IL-10, IL-1Ra (OOO Cytokine, Russia) were determined in oral fluid samples of the subjects examined by the ELISA method; Lysozyme is a turbidimetric method. The use of a probiotic rinse aid in the subjects of group 1 did not affect the level of cytokines, but led to an increase in the content of lysozyme. It was found that the subjects of the 2 group showed a decrease in the level of IgG and proinflammatory cytokines, especially IL-17, IL-6, TNFα, as well as an increase in the anti-inflammatory cytokine IL-1Ra. The results obtained revealed the anti-inflammatory effect of the probiotic mouthwash "ImmunitDominos", which allows it to be recommended as a preventive agent that increases the local protection of the oral cavity.