IMMUNOPHARMACOLOGICAL ASPECTS OF EXPERIMENTAL EVALUATION OF THE NEW BIOCOMPOUND

Abstract

The publication is devoted to very topical issues in the field of immunopharmacology on the creation of new bio compounds from the group of probiotics and biologically active substances produced by them, namely, the development of a new hepatoprotective pharmacological drug with an immunomodulatory effect. Today, in the field of modern pharmacology and biotechnology, microorganisms of the hay bacillus are promising and in-demand producers for obtaining a new class of bio compounds – metabiotics. One of the key advantages is the absence of cells in their composition, which significantly reduces the immune burden on the macroorganism while maintaining its functional and biological potential. At the same time, the issues of the development, creation and construction of medical immunobiological preparations of metabiotics for use in the pharmaceutical, medical and biotechnological fields remain very relevant and far unresolved. The purpose of the study: to experimentally study the immunotropic effect of a new bio compound on cellular immunity using a model of acute toxic hepatitis in laboratory animals. Materials and methods: the study used metabolites synthesized by hay bacillus, namely Bacillus subtilis strains B-23xx, B-99xx1 and B-99xx2. In our earlier studies, the safety was demonstrated and a significant hepatoprotective effect of the above strains was established, which can be successfully used to construct modern hepatopicts with hepatoprotective properties. At the same time, the unexplored possibility of systemic immunotropic action of these metabiotic compounds remains very relevant. Acute toxic liver damage was reproduced in white laboratory rats by single oral administration of a hepatotropic poison, carbon tetrachloride. The new bio compound was administered intragastrically in the form of a suspension. In the experiment, such indicators as quantitative determination of lymphocytes and antibody-forming cells, determination of phagocytic activity and metabolic activity of peripheral blood neutrophils were evaluated. Results: it was found that the simultaneous use of three complexes of biologically active substances can provide a significant potentiating immunotropic pharmacological effect. The phagocytic activity of neutrophils increased multiple times from 4.9 to 8.1 (depending on the study group). This was accompanied by a 5.3, 4.1, and 3.0-fold increase in the number of T and B lymphocytes. The dynamics of the increase in the number of these cells was also on the 12th-14th day of the experiment, with the indicators returning to the initial values on the 28th day. Discussion and conclusions: as a result of the performed studies, it was found that the proposed hepatoprotective bio compounds also have an immunomodulatory effect, which makes it possible to recommend it for the design of new and promising medicinal candidates for the group of medical immunobiological drugs.