ANTICITOKINE ACTIVITY OF MICROORGANISMS ISOLATED AT CHRONICAL BACTERIAL PROSTATITE

Abstract

The purpose of the present study is to evaluate the anticytokine activity of opportunistic micro-organisms isolated from the secretion of the prostate gland in patients with chronic bacterial prostatitis (CKD).

Materials and methods. In vitro tests were conducted on 50 strains of pathogenic microorganisms of different species isolated from the secret of the prostate gland in men who complained of chronic bacterial prostatitis and confirmed diagnosis. Isolated micro-organisms included different staphylococcus species (Staphylococcus aureus, S. haemolyticus, S. Epidermidis), Enterococcus faecalis и Escherichia coli.

To assess the anti-cytokine activity (ATA) of bacteria in relation to cytokines of IL-4, IL-6, IL-8, IL-17A and necrosis factor of tumor alpha (TNF α), an immunoenzyme method was used using sets of Cytokine SLR (St. Petersburg).

Results. The obtained data demonstrated that the anticytokine sign is widespread among various types of conditionally pathogenic micro-organisms isolated from the secret of the prostate gland in patients with chronic bacterial prostatitis. In particular, it was established that the isolates of S. aureus show statistically significant differences in the level of ASA in relation to IL-6 and IL-17A, and the culture of S. haemolyticus - in relation to IL-4 compared to other studied micro-organisms. At the same time, the expression of anti-cytokine activity in relation to IL-8 and TNF α in bacteria of different species did not quite differ.

Conclusions.  Anticytokine activity can be considered as an important marker of bacterial survival under conditions of prolonged interaction with factors of local host immunity in chronic bacterial prostatitis.

Further studies in this direction can give new practically useful insights, including those relating to the development of new approaches to the diagnosis of chronic bacterial prostatitis.