EFFECT OF BURSA FABRICIUS EXTRACT ON IMMUNOLOGICAL PARAMETERS OF BLOOD OF C57BL/6 MICE WITH CYCLOPHOSPHAMIDE-INDUCED IMMUNODEFICIENCY

Abstract

Cyclophosphamide is widely used anticancer and immunosuppressive drug. One of its negative side effects is the myelodepression and leukopenia. A natural and synthetic substances are able to reduce such negative effects. Protective effects of antioxidants on bone marrow are known when used together with cyclophosphamide. Plant and animal active peptides are also of interest due to their low toxicity, broad-spectrum biological activity. The "Bursanatal" due to the content of active peptides, growth factors, cytokines, antioxidants also have prospects for use as an immunomodulator, and has previously shown a positive effect on immunity under infectious diseases in farm animals.

The aim of this study was to investigate the effect of the "Bursanatal" on immunological parameters of the blood in C57Bl/6 mice with cyclophosphamide-induced immunosuppression.

Methods. The extract of the bursa was obtained from chickens aged 35-42 days by homogenization and subsequent removal of the heat-labile fraction. The final extract contains biologically active substances ranging from 1 to 10 kDa in size. Immunodeficiency was induced with single injection of cyclophosphamide (200 mg / kg body weight). The experimental group of mice received the bursa extract intraperitoneally for 7 days (0.1 ml / 20 mg body weight). After 7 days, the mice were weighted, hematological analysis was performed using hematological analyzer and flow cytometry (T- and B-lymphocyte subpopulations).

Results. Cyclophosphamide-treated mice developed B-cell-lymphopenia. After the bioorganocomplex exposure B-cell increase was observed in the peripheral blood compared to the control. A change in the ratio of T-lymphocyte subpopulations was also observed, which was reflected in their total relative concentration in the blood.

Conclusions. The obtained data allow to suggest the absence of a pronounced immunotoxic effect of the “Bursanatal”. Moreover the active ingredients of the extract are good candidates for alternative adjuvant chemotherapy to reduce immunotoxicity.