ANALYSIS OF LEVEL EXPRESSION OF THE HUMAN GENE TIM-1 USING REAL-TIME QUANTITATIVE PCR

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Abstract

The expression level of a human T-cell immunoglobulin-mucin domain (TIM-1) gene is diagnostically signifi cant in human diseases. The purpose of this work was to develop a real-time PCR method for analysis the expression levels of the human TIM-1 gene. The design of primers and fl uorescent probe on the conservative parts of the TIM-1 gene was carried out. The fragment of the TIM-1 gene was cloned into a plasmid and further it was used as a positive control. The optimization of the PCR conditions was performed using the HEK293 cell culture as a model. The expression levels of the TIM-1 gene were determined in the saliva of ten healthy volunteers and they did not diff er signifi cantly. The system being developed may be an alternative to closed commercial systems. We plan to use it in the research of diff erent pathologies in the future.

About the authors

T. Yu. Bondarenko

FBUN State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor

Author for correspondence.
Email: lemtat@ngs.ru

PhD, Researcher of Laboratory of Molecular Epidemiology EDI, 

Koltsovo, Novosibirsk Region

Russian Federation

V. A. Svyatchenko

FBUN State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor

Email: fake@neicon.ru

PhD, Head of Laboratory of virology of flaviviruses, 

Koltsovo, Novosibirsk Region

Russian Federation

V. A. Ternovoi

FBUN State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor

Email: fake@neicon.ru

PhD, Head of Laboratory of Molecular Epidemiology EDI,

Koltsovo, Novosibirsk Region

Russian Federation

References

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  2. Feigelstock D., Thompsonv P., Mattoov P., Zhang Y., Kaplan G. G. The human homolog of HAVcr-1 codes for a hepatitis A virus cellular receptor. J Virol. 1998 Aug;72(8):6621–8
  3. Chae S. C., Song J. H., Lee Y. C., Kim J. W., Chung H. T. The association of the exon 4 variations of Tim-1 gene with allergic diseases in a Korean population. Biochem Biophys Res Commun. 2003 Dec 12; 312(2):346–50.
  4. Moresco R. N., Bochi G. V., Stein C. S., De Carvalho J. A. M., Cembranel B. M., Bollick Y. S. Urinary kidney injury molecule-1 in renal disease. Clin Chim Acta. 2018 Dec;487:15–21.
  5. Bondarenko T. Y., Ternovoi V. A., Svyatchenko V. A., Kiselev N. N., Chausov E. V., Muntyanova M. U., Nemtsov Y. V., Yashin V. V., Kryuk N. I., Kuslii A. G., Nikulin L. G., Netesov S. V. Complete genomic sequence of rapidly replicating strain MB-7 of hepatitis a virus and its characterization in comparison with nucleotide sequence of other hepatitis a virus strain. Mol. Genet. Microbiol. Virol. 2010; 25: 39–46.

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Copyright (c) 2019 Bondarenko T.Y., Svyatchenko V.A., Ternovoi V.A.

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