THE STUDY OF ABILITY OF DENDRITIC CELLS LOADED WITH AUTOLOGICAL TUMOR LIZATE OF PATIENT SWITHHIGH-GRADE GLIOMA TO ACTIVATE ANTIGEN-SPECIFIC T-CELL RESPONSE IN VITRO

E. D. Soloveva; Соловьева Е. Д.; T. V. Tyrinova; Тыринова Т. В.; A. V. Kalinovsky; Калиновский А. В.; S. V. Mishinov; Мишинов С. В.; S. V. Chernov; Чернов С. В.; V. V. Stupak; Ступак В. В.

doi:10.31857/S102872210006537-5

THE STUDY OF ABILITY OF DENDRITIC CELLS LOADED WITH AUTOLOGICAL TUMOR LIZATE OF PATIENT SWITHHIGH-GRADE GLIOMA TO ACTIVATE ANTIGEN-SPECIFIC T-CELL RESPONSE IN VITRO

Authors: Soloveva E.D.¹, Tyrinova T.V.², Kalinovsky A.V.³, Mishinov S.V.⁴, Chernov S.V.³, Stupak V.V.⁴
Affiliations:
1. Novosibirsk state university
2. Institute of Fundamental and Clinical Immunology
3. Federal Neurosurgical Center, Ministry of Health of the Russian Federation
4. «Novosibirsk Research Institute of Traumatology and Orthopedics them. Y. L. Tsivyana», Ministry of Health of the Russian Federation
Issue: Vol 22, No 2-2 (2019)
Pages: 933-935
Section: ORIGINAL ARTICLES
Submitted: 24.07.2020
Accepted: 24.07.2020
Published: 15.07.2019
URL: https://rusimmun.ru/jour/article/view/410
DOI: https://doi.org/10.31857/S102872210006537-5
ID: 410

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Abstract

In the present work we demonstrated that IFNα-induced dendritic cells (IFN-DCs) loaded with a lysate of autologous tumor cells of high-grade glioma patients (DC_LYS) more eff ectively stimulated the proliferative response of autologous T-cells compared with unloaded IFN-DCs (DC_LPS) and did not induce apoptosis of CD4⁺ and CD8⁺ T cells. Moreover, DC_LYS activated cytotoxic CD8⁺ T cells (in the degranulation test) in cultures of autologous mononuclear cells of studied patients.