INFLUENCE OF OPSONINS OF FRESH AND INACTIVATED UNDER 56 °C BLOOD SERUM ON ZYMOSAN-INDUCED PRODUCTION OF REACTIVE OXYGEN SPECIES BY RAT LEUCOCYTES

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Abstract

The production of reactive oxygen species by blood leukocytes and bone marrow cells in the reaction of luminol-dependent chemiluminescence under the action of non-opsonized zymosan and zymosan opsonized with fresh or inactivated autologous serum at 56 °C was investigated. It was established that an increase in the chemiluminescence levels takes place in all three variants of phagocyte activation, but it is most pronounced when using fresh serum.

About the authors

Ju. I. Shilov

“Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” – a branch of the PFRC UB RAS;
Acad. E. A. Wagner Perm State Medical University

Author for correspondence.
Email: jshilov@mail.ru

PhD, MD, Associate Professor, Leading Researcher,

Perm

Russian Federation

A. P. Godovalov

Acad. E. A. Wagner Perm State Medical University

Email: fake@neicon.ru

PhD, MD, Leading Researcher of Central Scientifi c Research Laboratory and Associate Professor of the Department of Microbiology and Virology, 

Perm

Russian Federation

D. Ju. Shilov

LLC MC “Family Doctor”

Email: fake@neicon.ru

PhD, MD, Allergist-Immunologist, Leading Specialist,

Moscow

Russian Federation

S. Ju. Shilov

“Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” – a branch of the PFRC UB RAS;
Acad. E. A. Wagner Perm State Medical University

Email: fake@neicon.ru

PhD, MD, Junior Researcher,

Moscow

Russian Federation

D. Eliáš

Charles University

Email: fake@neicon.ru

medical student, Faculty of Medicine in Hradec Kralove,

Hradec Kralove

Russian Federation

References

  1. Makni-Maalej K., Chiandotto M., Hurtado-Nedelec M., Bedouhene S., Gougerot-Pocidalo M. A., Dang P. M., ElBenna J. Zymosan induces NADPH oxidase activation in human neutrophils by inducing the phosphorylation of p47phox and the activation of Rac2: involvement of protein tyrosine kinases, PI3Kinase, PKC, ERK1/2 and p38MAPkinase. Biochem Pharmacol. 2013, 85(1), 92–100.
  2. Morris M. R., Dewitt S., Laffafian I., Hallett M. B. Phagocytosis by inflammatory phagocytes: experimental strategies for stimulation and quantification. Methods Mol. Biol. 2003, 225, 35–46.
  3. Mankovich A. R., Lee C. Y., Heinrich V. Differential effects of serum heat treatment on chemotaxis and phagocytosis by human neutrophils. PLoS One 2013, 8(1), e54735. doi: 10.1371/journal.pone.0054735.

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Copyright (c) 2019 Shilov J.I., Godovalov A.P., Shilov D.J., Shilov S.J., Eliáš D.

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