RECOMBINANT PNEUMOLYSIN INDUCES THE FORMATION OF ANTIBODIES AND PROTECTS MICE FROM PNEUMOCOCCAL INFECTION

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Abstract

The obtained recombinant pneumolysin (rPLY) Streptococcus pneumoniae in atoxic form. The immunogenic and protective activity of rPLY was studied. It was shown that in the sera of mice after double injection of rPLY, the IgG titer increased by up to 32 times as compared with the sera of intact mice. After the third administration of rPLY, the IgG titer increased by up to 15 times compared with the initial IgG level after the second immunization. The protective activity of rPLY revealed in the experience of active protection in intraperitoneal infection of S. pneumoniae mice.

About the authors

D. S. Vorobyev

Mechnikov Research Institute for Vaccines and Sera

Author for correspondence.
Email: vorobievdenis@yandex.ru

PhD, Senior researcher of Laboratory of therapeutic vaccines,

Moscow

Russian Federation

E. S. Petukhova

Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

Aspirant of Laboratory of therapeutic vaccines,

Moscow

Russian Federation

A. V. Sidorov

Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

PhD, Head of the Laboratory of genetics of DNA-containing viruses,

Moscow

Russian Federation

I. B. Semenova

Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

D. Sc., Leading researcher of Laboratory of therapeutic vaccines,

Moscow

Russian Federation

Yu. V. Volokh

Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

Junior researcher of Laboratory of therapeutic vaccines,

Moscow

Russian Federation

A. Yu. Leonova

Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

Junior researcher of Laboratory of microbiology of conditionally pathogenic bacteria,

Moscow

Russian Federation

N. A. Mikhailova

Mechnikov Research Institute for Vaccines and Sera

Email: fake@neicon.ru

D. Sc., Head of the Laboratory of protective antigens,

Moscow

Russian Federation

References

  1. Subramanian K., Neill D. R., Malak H. A., Spelmink L., Khandaker S., Dalla Libera Marchiori G., Dearing E., Kirby A., Yang H., Achour A., Nilvebrant J., Nygren P. A., Plant L., Kadioglu A., Henriques-Normark B. Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nat. Microbiol. 2019, 4(1): 62–70.
  2. Ашмарин И. П., Воробьев А. А. Статистические методы в микробиологических исследованиях. Ленинград, 1962: 180 С.
  3. Гланц С. А. Медико-биологическая статистика. Изд-во «Практика». Москва, 1999: 459 С.

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Copyright (c) 2019 Vorobyev D.S., Petukhova E.S., Sidorov A.V., Semenova I.B., Volokh Y.V., Leonova A.Y., Mikhailova N.A.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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