Email this article Evaluation of triterpenoid miliacin-related anti-mutagenic effect in mice exposed to ionising radiation
- Authors: Sarycheva Y.A.1, Tokareva A.A.1, Shtil A.A.2, Kolyvanova M.A.3, Morozov V.N.3, Panfilova T.V.1, Frolov B.A.1
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Affiliations:
- Orenburg State Medical University
- Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology
- Russian State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical-Biological Agency
- Issue: Vol 23, No 2 (2020)
- Pages: 153-156
- Section: SHORT COMMUNICATIONS
- URL: https://rusimmun.ru/jour/article/view/558
- DOI: https://doi.org/10.46235/1028-7221-331-EOT
- ID: 558
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Abstract
Triterpenoid miliacin-related anti-mutagenic effect aftert exposure to isonizing radiation was studied to assess its protective properties against radiation-induced immunosuppression. (CBAxC57Bl6)F1 mice were subdivided into four groups: 1) intact mice; 2) irradiated mice; 3) miliacin-pretreated irradiated mice; and 4) miliacin-solvent-pretreated irradiated mice. Irradiation of animals was performed on the X-ray device “RUST-M1” (4 Gy; exposure time 288 sec.). Twenty four hours post irradiation myelokaryocytes were isolated for analysis. Chromosome preparations were examined by light microscopy. Injections of miliacin (4 mg/kg daily for 3 consecutive days) attenuated the irradiation-triggered mutagenic effect assessed by counting cells with chromosomal aberrations as well as number of aberrations per 100 metaphase plates. Miliacin exerted a protective effect on radiation-induced chromosomal aberrations, although degree of protection was less pronounced compared to chemically induced mutagenesis. These data indicate about limited potential for miliacin to protect central immune organs such as red bone marrow upon radiation exposure.
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About the authors
Yu. A. Sarycheva
Orenburg State Medical University
Author for correspondence.
Email: djsarycheva1985@mail.ru
Sarycheva Yuliya A. - PhD (Medicine), Associate Professor, Department of Pathological Physiology
460000, Orenburg, Park ave., 7
Phone: 7 (922) 550-47-57
Russian FederationA. A. Tokareva
Orenburg State Medical University
Email: fake@neicon.ru
Assistant Professor, Department of Pathological Physiology
Orenburg
Russian FederationA. A. Shtil
Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology
Email: fake@neicon.ru
PhD, MD (Medicine), Head, Laboratory of Tumor Cell Death
Moscow
Russian FederationM. A. Kolyvanova
Russian State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical-Biological Agency
Email: fake@neicon.ru
Head, Department of Medical Physics and Radiation Technologies
Moscow
Russian FederationV. N. Morozov
Russian State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical-Biological Agency
Email: fake@neicon.ru
Research Associate, Department of Medical Physics and Radiation Technologies
Moscow
Russian FederationT. V. Panfilova
Orenburg State Medical University
Email: fake@neicon.ru
PhD (Medicine), Associate Professor, Department of Pathological Physiology
Orenburg
Russian FederationB. A. Frolov
Orenburg State Medical University
Email: fake@neicon.ru
PhD, MD (Medicine), Professor, Head, Department of Pathological Physiology
Orenburg
Russian FederationReferences
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