Evaluation of triterpenoid miliacin-related anti-mutagenic effect in mice exposed to ionising radiation

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Abstract

Triterpenoid miliacin-related anti-mutagenic effect aftert exposure to isonizing radiation was studied to assess its protective properties against radiation-induced immunosuppression. (CBAxC57Bl6)F1 mice were subdivided into four groups: 1) intact mice; 2) irradiated mice; 3) miliacin-pretreated irradiated mice; and 4) miliacin-solvent-pretreated irradiated mice. Irradiation of animals was performed on the X-ray device “RUST-M1” (4 Gy; exposure time 288 sec.). Twenty four hours post irradiation myelokaryocytes were isolated for analysis. Chromosome preparations were examined by light microscopy. Injections of miliacin (4 mg/kg daily for 3 consecutive days) attenuated the irradiation-triggered mutagenic effect assessed by counting cells with chromosomal aberrations as well as number of aberrations per 100 metaphase plates. Miliacin exerted a protective effect on radiation-induced chromosomal aberrations, although degree of protection was less pronounced compared to chemically induced mutagenesis. These data indicate about limited potential for miliacin to protect central immune organs such as red bone marrow upon radiation exposure.

About the authors

Yu. A. Sarycheva

Orenburg State Medical University

Author for correspondence.
Email: djsarycheva1985@mail.ru

Sarycheva Yuliya A. - PhD (Medicine), Associate Professor, Department of Pathological Physiology

460000, Orenburg, Park ave., 7

Phone: 7 (922) 550-47-57

Russian Federation

A. A. Tokareva

Orenburg State Medical University

Email: fake@neicon.ru

Assistant Professor, Department of Pathological Physiology

Orenburg

Russian Federation

A. A. Shtil

Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center of Oncology

Email: fake@neicon.ru

PhD, MD (Medicine), Head, Laboratory of Tumor Cell Death

Moscow

Russian Federation

M. A. Kolyvanova

Russian State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical-Biological Agency

Email: fake@neicon.ru

Head, Department of Medical Physics and Radiation Technologies

Moscow

Russian Federation

V. N. Morozov

Russian State Research Center – Burnasyan Federal Medical Biophysical Center of Federal Medical-Biological Agency

Email: fake@neicon.ru

Research Associate, Department of Medical Physics and Radiation Technologies

Moscow

Russian Federation

T. V. Panfilova

Orenburg State Medical University

Email: fake@neicon.ru

PhD (Medicine), Associate Professor, Department of Pathological Physiology

Orenburg

Russian Federation

B. A. Frolov

Orenburg State Medical University

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Head, Department of Pathological Physiology

Orenburg

Russian Federation

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Copyright (c) 2020 Sarycheva Y.A., Tokareva A.A., Shtil A.A., Kolyvanova M.A., Morozov V.N., Panfilova T.V., Frolov B.A.

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