Experience of applying flow cytometry to assess immunologic reactivity in subjects with increased blood vanadium concentration

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Abstract

Environmental pollution with external vanadium results in its accumulation in human biological media and alters ability of human body to properly react to negative impact after exposure to haptens. Aim of the work was to assess immunologic reactivity in subjects bearing increased blood vanadium (hapten) level by using flow cytometry. There were examined adults bearing various blood vanadium concedntration. The test group was comprised by adult subjects (n = 50) having blood vanadium concentration exceeding that one for the upper limit of the reference level and mean reference group (n = 42) corresponding to reference range by 3.7- (р < 0.001) and 4.3-fold, respectrively, It was found that subjects with excessively vanadium-contaminated serum were featured with stronger lymphocytes activation, increased count of effector helper T cells, lowered count of regulatory lymphocytes and decreased humoral immune arm, and suppressed p53-dependent control of cellular cycle. Thus, flow cytometry allows to assessing immunologic reactivity in subjects with varying level of blood exogenous chemicals as well as determining body adaptation potential to ewffects exerted by environmental chemical cues.

About the authors

D. G. Dianova

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; Perm State Pharmaceutical Academy

Email: fake@neicon.ru

PhD, MD (Medicine), Associate Professor, Senior Research Associate, Department of Immunobiological Methods of Diagnostics; Associate Professor, Department of Pharmacology

Perm

Russian Federation

O. V. Dolgikh

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; Perm National Research Polytechnic University

Author for correspondence.
Email: oleg@fcrisk.ru

Dolgikh Oleg V. - PhD, MD (Medicine), Head, Department of Immunobiological Methods of Diagnostics; Professor, Department of Human Ecology and Life Safety

614045, Perm, Monastyrskaya str., 82

Phone: 7 (342) 236-39-30

Russian Federation

V. B. Alekseev

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: fake@neicon.ru

PhD, MD (Medicine), Director

Perm

Russian Federation

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Copyright (c) 2020 Dianova D.G., Dolgikh O.V., Alekseev V.B.

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