NEUTROPHILS AS THE CYTOKINE PRODUCING CELLS



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Abstract

In work secretion by neutrophils the preforming of cytokins after an hour incubation to the RPMI 1640 environment is studied. Secretion of cytokin was defined with use of multiplex sets of the BioRad company (USA) for definition of 17 cytokin (G-CSF, GM-CSF, IL-10, IL-12p70, INF-γ, IL-13, IL-17A, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, TNF-α, IL-8, MCP-1, MIP-1β) on the MAGPIX-100 device (USA). It is established that at an incubation of neutrophils in the environment of RPMI 1640 within 1 hour in the environment of an incubation (supernatant) come concentration of which in a supenatant of neutrophils exceeded GM-CSF, IL-12p70, IL-17A, IL-1β, IL-6 IL-8 and MIP-1β reference level (RPMI-1640 environment) by 1.2–7.0 times (p <0.05–0.001). Thus, neutrophils are able to secrete preformed cytokines into the culture medium for 1 hour after the start of their incubation.

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About the authors

A. V. Zurochka

Institute of Immunology and Physiology UrB RAS;
South-Ural State University (National Research University)

Author for correspondence.
Email: av_zurochka@mail.ru

leader scientific researcher of the laboratory of immunology of inflammation;

MD, professor of the Department of food and biotechnology, senior researcher of the laboratory of molecular immunology,

Chelyabinsk

Russian Federation

V. A. Zurochka

Institute of Immunology and Physiology UrB RAS;
South-Ural State University (National Research University)

Email: fake@neicon.ru

senior scientific researcher of the laboratory of immunology of infl ammation;

MD, professor of the Department of food and biotechnology, senior researcher of the laboratory of molecular immunology,

Chelyabinsk 

Russian Federation

M. A. Dobrynina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

unior scientific researcherof the laboratory of immunology of inflammation,

Chelyabinsk 

Russian Federation

L. O. Fomina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

post-graduate student of the Institute of immunology and physiology,

Chelyabinsk 

Russian Federation

A. I. Fayzullina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

post-graduate student of the Institute of immunology and physiology,

Chelyabinsk 

Russian Federation

V. A. Gritsenko

Orenburg Federal Research Сenter UrB RAS

Email: fake@neicon.ru

MD, professor, chief researcher,

Chelyabinsk 

Russian Federation

References

  1. Долгушин И.И., Зурочка А. В., Чукичев А. В. Регуляторные пептиды нейтрофилов (нейтрофилокины). Иммунология. 1995, 4, 40–45.
  2. Зурочка А.В., Зурочка В. А., Зуева Е. Б., Добрынина М. А., Дукарт В. В., Черкасов С. В, Гриценко В. А. Влияние синтетического пептида активного центра гранулоцитарно-макрофагального колониестимулирующего фактора (ГМ-КСФ) на продукцию цитокинов нейтрофилами периферической крови человека. Бюллетень Оренбургского научного центра УрО РАН. 2015. 4: 1–9. [Электронный ресурс].
  3. Зурочка А. В., Хайдуков С. В., Кудрявцев И. В., Черешнев В. А. Проточная цитометрия в биомедицинских исследованиях. Екатеринбург: РИО УрО РАН, 2018, 720 с.
  4. Хайдуков С.В., Байдун Л. А., Зурочка А. В., Тотолян Арег. А. Стандартизованная технология «Исследование субпопуляционного состава лимфоцитов периферической крови с применением проточных цитофлюориметров-анализаторов». Российский иммунологический журнал. 2014. Т. 8 (17). 4: 974–992.
  5. Wong L., Wilson R. D. The indentification of Fc and C3 receptors on human neutrophils. J. Immunol. Meth. 1975. 7: 69–76.

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Copyright (c) 2019 Zurochka A.V., Zurochka V.A., Dobrynina M.A., Fomina L.O., Fayzullina A.I., Gritsenko V.A.

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